表面聲波氣體及生化感測器研製與應用

Preparation and Application of Surface Acoustic Wave Gas- and Bio-Sensor

張宏維(Hung Wei, Chang)

指導教授 ： 施正雄

國立臺灣師範大學/理學院/化學系/博士(2007年)

摘要

本研究建立表面聲波氣體及生化感測器，以偵測有機氣體及生化物質，例如：蛋白質和胰島素。在本研究中研製一低價位的氣體表面聲波感測器以偵測微量有機氣體污染物，本研究亦建立剪力水平表面聲波液體感測器已偵測血紅素、肌紅素、麥膠蛋白及胰島素等。

氣相表面聲波感測器是利用低價位（<10美金）通訊用SAW晶片及頻率計數器和RS-232組裝而成，並自行撰寫電腦程式來做頻率訊號收集及數據收集處理此氣體表面聲波感測器用來偵測各種空氣污染物例如：有機酸、醛類、醇類、烷類、烯類及炔類等，並且利用這自組裝的表面聲波感測器所構成的多頻道系統也用來同時偵測各種不同的空氣污染物。

剪力水平表面聲波感測器塗佈有C60/蛋白質，例如C60/血紅素, C60/肌紅素，C60/榖膠蛋白，C60/抗胰島素等，分別來偵測水溶液中特定待測物，例如：抗血紅素抗體，抗肌紅素抗體，抗榖膠蛋白抗體，以及胰島素。血紅素和肌紅素分別與C60之間的作用力及固定化是利用塗佈有C60的剪力水平表面聲波感測器對血紅素和肌紅素吸附所引起的頻率變化只有部分回復的現象以及FT-IR光譜中有新的吸收峰產生來判斷，C60與血紅素之間有化學鍵的生成，利用這樣的化學鍵生成來把水溶液中的血紅素固定在剪力水平表面聲波感測器表面上。而利用這樣固定有C60/血紅素或者C60/肌紅素的剪力水平表面聲波感測器來分別偵測水溶液中的抗血紅素和抗肌紅素抗體，兩者的靈敏度分別是0.14 和1.27 kHz/(?g/mL)，偵測下限分別是0.32 以及 0.035 ?g/mL。此外，雙頻道塗佈C60-Hb及C60-Mb表面聲波感測器亦被以研製以同時偵測血紅素和肌紅素抗體。
而塗佈有C60/抗胰島素抗體的剪力水平表面聲波感測器亦被研製並用來偵測水溶液中的胰島素。此剪力水平表面聲波胰島素感測器在人體胰島素濃度範圍內，感測器的頻率感應訊號和胰島素濃度有良好線性關係及很好的靈敏度（130 Hz/pM），而感測器的偵測下限是0.58pM。

關鍵字

剪力水平表面聲波 ； 碳六十 ； 免疫感測器 ； 胰島素 ； HASH(0x8a496d8) ； HASH(0x8a49750)

並列摘要

Surface acoustic wave gas and Bio-sensors were developed to detect organic gases and biospecies, e.g. proteins and insulin. An inexpensive surface acoustic wave (SAW) sensor system was developed and used to detect trace pollutants in the air. For liquid environment, a shear-horizontal surface acoustic wave sensor system was established to apply in immunosensor system to detect various samples, e.g. hemoglobin, myoglobin, gliadin, and insulin, in aqueous solution.

The homemade SAW gas sensor is composed of a low cost (< 10 $USD) SAW chip for correspondence, an inexpensive counter and RS-232 computer interface with a written computer program for frequency signal acquisition and data processing. With different adsorbent coatings, various SAW gas sensors were prepared to detect organic pollutants, e.g. carboxylic acids, aldehydes, alchohols, alkanes, alkenes, alkynes in the air. Furthermore, the multi-channel SAW gas detection system was also developed to detect various air pollutants simultaneously.

Shear horizontal surface acoustic wave sensors immobilized with C60/proteins, e.g. C60/hemoglobin(C60-Hb), C60/myoglobin(C60-Mb), and C60/gliadin, and C60/anti-insulin coatings were prepared and applied to detect specific antibodies, e.g. anti-hemoglobin, anti-myoglobin, anti-gliadin, insulin, respectively, in liquid environments. The immobilizations of hemoglobin and myoglobin onto fullerene were studied through a C60-coated SH-SAW sensor system in liquid. The partially irreversible responses for these proteins were observed by the desorption study, which implied that fullerene could chemically react with these proteins. Both C60-Hb and C60-Mb coating materials were successfully prepared and identified with an FTIR spectrometer. The C60-Hb and C60-Mb coated SH-SAW immunosensors exhibited linear frequency responses to the concentration of anti-Hb and anti-Mb antibodies with sensitivities of 0.14 and 1.27 kHz/ (?g/mL), respectively. Both C60-protein coated SH-SAW immunosensorms showed detection limits of 0.32 and 0.035 ?g/mL for anti-Hb and anti-Mb antibodies, respectively, in aqueous solution. In addition, dual channel SAW immunosensors coated with C60-Hb and C60-Mb were prepared and applied to detect anti-Hb and anti-Mb antibodies simultaneously.

An immobilized fullerene C60/anti-insulin antibody was prepared and applied in shear horizontal surface acoustic wave (SH-SAW) immunosensors to detect insulin in aqueous solutions. Within the range of normal human insulin concentration, the SH-SAW immunosensors immobilized with C60/anti-insulin coating exhibited linear frequency responses to the concentration of insulin with sensitivity of 130 Hz/pM. The SH-SAW immunosensor with C60/anti-insulin coating showed detection limit of 0.58 pM for insulin in aqueous solution.

並列關鍵字

Shear horizontal surface acoustic wave ； SH-SAW ； C60 ； Fullerene ； Immunosensor ； insulin

參考文獻

1. 張景裕, 張獻彰, 微奈米生物感測器系統在生物醫學的應用, 科儀新知28 (2006) 17-25.

3. 田蔚城, 生物技術的發展與應用, 1997

2. P. B. Luppa, L. J. Sokoll, D. W. Chan, Immunosensors—principles and applications to clinical chemistry Chinica Chimica Acta 314 (2001) 1-26.

4. S. Michael, D. Panos, L. Nickolay, T. Christopher, Microcantilever Transducers: A New Approach in Sensor Technology, Anal. Chem. 1 (2002) 569A-575A.

5. R. Raiteri, M. Grattarola, H.J. Butt, P. Skladal, Micromechanical cantilever based biosensors, Sens. Actuators, B, Chem. 79 (2001) 115-126.