光學影像技術於生物組織之研究

The study of optical imaging technique on biological tissues

戴川義(Chuan-Yi Tai)

指導教授 ： 李亞儒

國立臺灣師範大學/理學院/光電科技研究所/碩士(2011年)

摘要

光學影像技術具有價格低廉、快速顯像、可攜性等優點。本研究可分為兩部分，首先，我們建立一套頻域式光學同調斷層攝影系統，用以取得生物組織的背向散射訊號。其利用同調光源於異質性之組織，藉由獲得組織其不同折射率之背向散射訊號，致使重建組織之結構影像。
最後，我們有效利用都普勒光學微血管攝影術於仿體以及老鼠表皮血管分佈之成像。此外，並利用此技術於老鼠大腦之血管攝影實驗，使有效地成像出鼠腦之血管分佈影像。

關鍵字

頻域式光學同調斷層攝影系統 ； 都普勒光學微血管攝影術

並列摘要

Optical imaging technique has the advantages of cost-effectiveness, non-invasive, rapid imaging and portability.
In this thesis, we have two topics. Firstly, we developed a spectral-domain optical coherence tomography system to obtain biological tissue scattering, the system uses coherence gating of backscattered light for tomographic imaing of tissue structure. Variation in tissue scattering due to in-homogeneities in the optical index of refraction provide imaing contrast.
Finally, we have successfully used Doppler optical micro-angiography (DOMAG) to image phantom flow and skin blood perfusion in mice. We then conduct in vivo experiments on a mouse brain to demonstrate that DOMAG is capable of quantifying the blood flow within cerebrovascular network.

並列關鍵字

Spectral-domain optical coherence tomography system ； Doppler optical micro-angiography

參考文獻

[1]Wolfgang Drexler and James G. Fujimoto, “Optical Coherence Tomography Technology and Applications” Berlin, Heidelberg : Springer Berlin Heidelberg, 2008.

[2]Joseph A. Izatt, Manish D. Kulkarni, Siavash Yazdanfar, Jennifer K. Barton, and Ashley J. Welch, “In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography,” OPTICS LETTERS, 22, 1439-1441, 1997.

[3]Yonghua Zhao, Zhongping Chen, Christopher Saxer, Shaohua Xiang, Johannes F. de Boer, and J. Stuart Nelson, “Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity, ” OPTICS LETTERS, 25, 114-116, 2000.

[5]Ruikang K Wang, “Fourier domain optical coherence tomography achieves full range complex imaging in vivo by introducing a carrier frequency during scanning,” IOP PUBLISHING Ltd , PHYSICS MEDICINE BIOLOGY, 2007.

[6]Lin An, and Ruikang K. Wang, “Use of a scanner to modulate spatial interferograms for in vivo full-range Fourier-domain optical coherence tomography,” Optical Society of America, Vol. 32, No. 23, 2007.