抗氧化劑增補對激烈運動及魚油引發之氧化壓力的影響

姚承義

指導教授 ： 謝伸裕

國立臺灣師範大學/運動與休閒學院/體育學系/博士(2001年)

摘要

無資料

關鍵字

激烈運動 ； 魚油 ； 氧化性傷害 ； 自由基 ； 抗氧化劑

並列摘要

Exercise can improve performance, fitness, and metabolism, as well as modify body-fat ratio. But high-intensity exercise may also induce active oxygen species (AOS). Fish oil, which is one of the ω-3 PUFA series that can decrease TG and influence other plasma lipids, appears to prevent arteriosclerosis and CHD, but some evidence suggests it increases the lipid peroxidation in the cell membrane. Fish oil would then seem to influence the antioxidant defense system that causes injury or disease. This study investigates the effects on the antioxidant defence system of combining high-intensity exercise with fish oil supplementation.
Thirty-three healthy males were randomly assigned into eight groups (20.3±1.4 yrs; 64.3±7.9 kg), which were given set combinations of dietary fish oil (9 g per day), exercise (intensity 85-90 % HRmax reserve), anti-oxident supplementation (500 mg Vitamin C and 400 IU Vitamin E per day), and placebos. The experiment lasted 4 weeks in total. Venous samples were obtained prior to exercise and within 5 min after. 4 venous samples were also taken from fish oil groups during the four-week period. Blood from all groups was analyzed for sesuperoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total glutathione (T-GSH), total antioxidant ability (TAA), malodialdehyde (MDA), vitamin C & E, TG, HDL-C, LDL-C from forearm vein. A Generalized Estimating Equations (GEE) method was used for data analysis. Result: in no group was there a significant change in the body weight and body composition of the subjects. In the fish oil groups SOD activity significantly increased 11.27 kU/g-HB（P<.05）. Fish oil and vitamin C & E groups saw a decrease in GSH-Px levels, which did not appear in the exercise groups. However, statistical evidence is not sufficient to draw solid conclusions in this area. MDA change increased significantly after exercise in both exercise and dietary fish oil groups (values: 0.12 and 0.27 mmole/ml [P<.05] respectively). The evidence shows that the dosage of fish oil decreased the values of LDL-C and TG (-4.61 mg/dl and -8.85 mg/dl [P <.05] respectively). Exercise increased LDL-C levels (+2.27 mg/dl). Following exercise in the fish oil group, t-GSH increased significantly (36.61Ug/ml [34.2%] [P<.05]). MDA increased significantly after exercise in both exercise-alone and exercise/fish oil groups (+0.12 and +0.27 mmole/ml [P<.05] respectively). Height intensity exercise and fish oil is a real factor that induced more oxidative stress in this study. There is not sufficient evidence to support the claim that oxidative stress decreases after antioxidant supplementation, although there is some indication that this may be true. More research is required. The study concludes that exercise and fish oil separately causes an increase in oxidative stress, and this increase is greater when they are combined. It is also conclude that antioxidant supplementation has no effect on oxidative stress, although it is possible that a larger dosage may produce such an effect.

並列關鍵字

high-intensity exercise ； fish oil ； oxidative stress ； lipids peroxidation ； antioxidants