氣相層析/液相層析/毛細管電泳法對九種色胺類化合物的分離與分析

Comparison of the separation of nine tryptamine standards based on gas chromatography, high performance liquid chromatography and capillary electrophoresis methods

王曼憬(Man-Juing Wang)

指導教授 ： 林震煌

國立臺灣師範大學/理學院/化學系/碩士(2007年)

摘要

本實驗以氣相層析、液相層析及毛細管電泳三種分離方法進行研究，實驗分析物為九種色胺類衍生物 (α-methyltryptamine, AMT；
N,N-dimethyltryptamine, DMT；5-methoxy-α-methyltryptamine, 5-MeO-AMT；N,N-diethyltryptamine, DET；
N,N-dipropyltryptamine, DPT；N,N-dibutyltryptamine, DBT；N,N-diisopropyltryptamine, DIPT；
5-methoxy-N,N-dimethyltryptamine, 5-MeO-DMT；
5-methoxy-N,N-diisopropyltryptamine, 5-MeO-DIPT)。這九種分析物被選作測試樣品，是?了用來比較它們相對的偵測靈敏度、選擇性、分離所需時間、分離所需成本與分離時先後出現的順序等。
實驗結果發現，當S/N = 3時，氣相層析質譜法與液相層析/紫外光吸收法得到的偵測極限分別為0.5 ~ 15與0.3 ~1 μg/mL。相對於這兩種方法，使用毛細管區帶電泳/紫外光吸收法時，偵測極限可改善至0.4 ~ 1 μg/mL。當引用線上掃集-微胞電動的技術時，偵測極限甚至於還可大幅改善至2 ~ 8 ng/mL。
至於分離九種色胺類衍生物所需的時間，使用氣相層析法時，分離時間為11 ~ 15分鐘；使用液相層析法時，分離時間為8 ~ 23分鐘；使用線上掃集-微胞電動技術則需要20 ~ 26分鐘。分析物出現的先後順序基本上依據其分子量的大小，例如DMT最先出現，然後才是DET和DPT，最後才是DBT。但是，當分析物為一級胺 (例如AMT與5-MeO-AMT )、同分異構物 (DIPT與DPT) 或有5-甲醚基的官能基(例如5-MeO-AMT、5-MeO-DMT或
5-MeO-DIPT) 時，分離條件改變時，順序也會略有變動。

關鍵字

色胺類

並列摘要

Nine tryptamines, including α-methyltryptamine (AMT),
N,N-dimethyltryptamine (DMT), 5-methoxy-α-methyltryptamine (5-MeO-AMT), N,N-diethyltryptamine (DET),
N,N-dipropyltryptamine (DPT), N,N-dibutyltryptamine (DBT), N,N-diisopropyltryptamine (DIPT),
5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT), and
5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) were selected as model compounds.
Comparisons of their sensitivity, selectivity, time, cost and the order of migration are described based on different separation techniques (GC,HPLC and CE, respectively). As a result, the limit of detection (S/N = 3) obtained by GC/MS and LC/UV-absorption ranged from 0.5 to 15 μg/mL
and 0.3 to 1.0 μg/mL, respectively. In contrast to this, based on the CZE/UV-absorption method, the limit of detection (S/N = 3) was determined to 0.4–1 μg/mL. However, when the sweeping-MEKC mode was applied, it dramatically improved to 2–8 ng/mL. In the case of GC, HPLC and CE, migration times of the nine standards ranged from 11 to 15 min and 8 to 23 min by GC and HPLC, respectively; ranged from 20 to 26 min by sweeping-MEKC. The order of migration of DMT, DET, DPT and DBT follows the molecular weight, whereas the order of migration of AMT and 5-MeO-AMT (primary amines), DIPT (an isomer of DPT) and 5-methoxy-tryptamines (5-MeO-AMT, 5-MeO-DMT and 5-MeO-DIPT) can be altered by changing the separation conditions.

並列關鍵字

tryptamine

參考文獻

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