探討1-Thio-β-D-glucose表面修飾螢光金奈米團簇於腦瘤細胞、大腸桿菌與線蟲代謝後之螢光變化

螢光金奈米團簇（Gold Nanoclusters, Au NCs）具有獨特的光學性質、易於進行表面修飾及良好的生物相容性等優點，使其具有生物顯影及生物感測之應用性。本研究利用穀胱甘肽做為還原劑製備出發橘紅色螢光之金奈米團簇，並以β-D硫代葡萄糖（1-Thio-β-D-glucose）修飾其表面，合成出表面修飾葡萄糖之螢光金奈米團簇（TGlu-Au NCs）。我們利用顯微注射及餵食的方式，使TGlu-Au NCs進入秀麗隱桿線蟲（C. elegans）體內，在線蟲的咽、體腔及肛門都能清楚看到TGlu-Au NCs的螢光成像。使用大腸桿菌（E. coli）觀察TGlu-Au NCs的螢光變化，得到大腸桿菌濃度上升會使TGlu-Au NCs螢光強度下降之結果，顯示TGlu-Au NCs做為生物感測器之潛力。另將腦瘤細胞之糖酵解酶取出與TGlu-Au NCs反應，發現TGlu-Au NCs的螢光強度隨著反應時間愈長而下降愈多，顯示腫瘤細胞發生糖解作用，證實TGlu-Au NCs可利用腦瘤細胞的瓦氏效應行標記之作用。

關鍵字

螢光金奈米團簇；表面修飾； 1-Thio-β-D-glucose ；螢光成像；生物感測；瓦氏效應

並列摘要

Fluorescent gold nanoclusters have been widely employed in the bioimaging and biosensing because of their unique optical properties, facile surface modification and high biocompatibility. In this study, we used L-Glutathione as a reductant to prepared fluorescent gold nanoclusters (Au NCs) and then the surface of Au NCs was modified with 1-Thio-β-D-glucose (TGlu-Au NCs). TGlu-Au NCs were respectively microinjected and fed into C. elegans to investigate their in vivo fluorescence imaging. The fluorescence of TGlu-Au NCs was easily visible in the lumen of the digestive system, extending from the pharynx to the anal region. Furthermore, the fluorescence changes of TGlu-Au NCs were studied with E. coli. TGlu-Au NCs at 0.4 (O.D.) showed higher decreasing rate of fluorescence intensity than that of TGlu-Au NCs at 0.27 (O.D.). In addition, the glycolytic enzyme of brain tumor cells (U-87 MG) was added into TGlu-Au NCs solution. After the reaction with the glycolytic enzyme, the fluorescence intensity of TGlu-Au NCs was decreased with the increase of time. The results confirmed the Warburg effect of TGlu-Au NCs in brain tumor cell.