Cartoon design is widely used in commercial activities and daily life. The author discovered that cartoon design is the privilege of children and a cultural and creative product across generations. The influence of physiological structure, acquired culture, popular trends, living environment, and educational background often caused the difference in aesthetic preference between genders. What kind of cartoon image features can break the gender barrier? What are the differences in the preferences of cartoon images among children of different genders? Is there an interaction between gender and form on preference? These are the purpose of the study.