生物微培養器研製及其應用於數位全像顯微活細胞觀測

Development of micro-incubator for living cell observation with digital holographic microscopy

葉仲禹(Yeh Chung- Yu)

指導教授 ： 鄭超仁

國立臺灣師範大學/科技與工程學院/光電工程研究所/碩士(2022年)

https://doi.org/10.6345/NTNU202200304

摘要

本研究為研製生物微培養器系統維持生物細胞存活，並運用數位全像顯微鏡(Digital Holography Microscope, DHM)進行長時間(72 hrs.)活細胞量測，為了達到長時間連續觀測活細胞，本論文研製出可以放在顯微系統上的微型生物培養器系統。研製之微型培養器解決了現階段常會遇到實務應用上的問題，例如：溫控加熱不均、體型過大，沒有氣體系統導致能培養的細胞種類有所限制、成本過高等等，此研究運用微型控制器、低電壓電路設計與3D列印的技術，可依照量測系統做客製化設計，且改善製作成本、具有安全性，也能提供多種細胞生長、分裂之環境的生物微培養器，搭配數位全像顯微系統進行長時間的細胞觀測與造影，本研究實驗結果可驗證此系統具實務可行性，並可應用於觀測細胞分裂等結果。

關鍵字

數位全像術 ； 生物微培養器 ； 活細胞量測

並列摘要

This study is to develop a biological micro-incubator system to maintain the survival of biological cells and to use Digital Holography Microscope (DHM) for long-term measurement of living cells. The common type of incubator used for human cell culture is carbon dioxide incubator. In order to continuously observe cells, this paper has developed a biological micro-incubator system that can be placed on DHM system. The developed micro-incubator solves the problems often encountered at this stage, such as uneven temperature control and heating, excessive body size, without gas system will limit the types of cells that can be cultured, high cost, etc. Using a micro-controller with low-voltage circuit design and the 3D printing technology has designed a biological micro-incubator that can be customized according to the measurement system and reduce the production cost. Also, it is safe and can provide an environment for a variety of cells to divide, and assist in the use of a digital holographic microscopy system perform long-term cell observations.

並列關鍵字

Digital Holography ； Micro-Incubator ； Living Cells Measurement

參考文獻

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