介電泳於細胞實驗之電場分析

Electric Field Analysis for the Application of Dielectrophoresis on Cellular Experiments

黃昱畯(Yu-Chun Huang)

指導教授 ： 陳恕行

崑山科技大學/工程學院/機械工程研究所/碩士(2006年)

摘要

分子生物學不但對於醫學治療有助益，對工程人員更可提供微系統設計的有用資訊。在所有的微粒子操控的技術中，介電泳具有一項特別優勢就是具有非侵入式的操控細胞內部的能力。介電泳產生非對稱的電偶極化，使細胞受電場力作用後，順電場梯度移動。此介電泳力之大小和方向依存於細胞和溶液的介電性的差異及加交流電場強度等參數。常應用於生物醫學領域的研究上，如細胞分離以及細胞對外界作用的反應。
本研究在理論上探討了電極排列的影響及受頻率影響的細胞介電性。在實驗上進行了酵母菌細胞的介電排列實驗及植物原生質的變形實驗。

關鍵字

細胞 ； 分子生物學 ； 介電泳

並列摘要

Molecular biology not only holds clues for medical treatments but also provide engineers with valuable information for designing miniature systems. Among micro-scale manipulation, dielectrophoresis possesses a unique edge: the ability to reach intracellular components non-invasively. Dielectrophoresis is the movement of particles caused by electrical polarization effects in non-uniform AC electric fields. The force responsible for this motion is also governed by the dielectric properties both of the suspending medium and of the particles, as well as the geometry of the electric field. Potential applications of dielectrophoresis in bioengineering research include cell separation and cellular responses to external stimuli.
This paper reports results of theoretical investigations on electrode geometry and frequency-dependent properties of particles. Experimental results of dieletrophoreiss on yeast cells and protoplast are also presented.

並列關鍵字

cell ； Dielectrophoresis ； Molecular biology

參考文獻

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