酵母菌電性檢測與分析

The Measuring Methodroloqy and Anaylsis of Yeast Cell Electric Properties

吳炯鋒(Jiong-Feng Wu)

指導教授 ： 林水木

共同指導教授 ： 王文榮

崑山科技大學/工程學院/機械工程研究所/碩士(2009年)

https://doi.org/10.6828/KSU.2009.00044

摘要

生物細胞及菌類的分離，傳統上採用離心方式，其缺點是無法連續大量操作。近年來，依據生物細胞的介電性，採用介電泳技術，使同時產生正負介電泳現象，使不同細胞或菌類來分離其效果顯著。此外，亦採用膜過濾，及利用了菌類電滲透(eleutrosmosis)原理來分離。可知菌類的電性研究及應用是非常重要的。本文針對酵母菌的電性量測原理作研究，提出一套量測方法，並配合顯微鏡觀察及Zeta電位儀量測，觀察酵母菌在不同電場及pH值溶液中的運動行為，並預測其靜電荷及求得stoke修正因子。

關鍵字

交流電場 ； 介電泳 ； 電泳

並列摘要

In convention, the centrifugal method is used to separate the bio-cells and bacterium. However, the technology of scale-up and continuous separation can not be established. In recently, based on the dielectricity of bio-cells and bacterium, the electrophoresis is successfully used for separation. The positive and negative electrophoreses are created so that different cells of bacterium can be continuously separated. In addition, the method by using membrane filtering is also applied for separation. The electro-osmosis principle is applied in this method. Obviously, the investigation of the electric properties of bio-cells and bacterium is very important. In this thesis, the measuring principle of the electric properties a yeast cell is investigated. A methodology for measuring the electric properties is established. Observe the amplitudes of yeast under different AC current frequencies and in different pH solutions. The zeta potential of yeast is measured by using the zeta potential meter. Therefore, the net charge can be calculated and the corresponding Stoke’s correction factor can be obtained.

並列關鍵字

Ac electric field ； Dielectrophoretic ； Electrophoresis

參考文獻

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