Polymer materials have been widely used in flexible displays, biomedicine, solar energy, etc.. As compared with other materials, one of their most different is that polymer materials have transition phenomena as their temperature increases. Among the transition temperatures of polymer materials, glass transition temperature (Tg) is the most important one. When temperature of polymer materials increase up to its Tg, all of its exhibiting properties including mechanical, dimensional, optics, and thermodynamics properties dramatically change. As a result, it is an important issue to develop an experimental technique to measure their Tg. The study aims to measure Tg based on the gray level variation of digital images. A projector was first utilized to respectively project parallel and grid styles images onto the polymer materials. The relation between variation of gray level of digital image and temperature was recorded to extract Tg. Finally, the measurement results were compared with those obtained by dynamic mechanical analyzer (DMA)