蜂王漿中癸烯酸的抗發炎活性分析及研究

The Anti-Inflammatory Activity of 10-HDA from Royal Jelly

威迪雅(Debora Arny Widowati)

指導教授 ： 彭及忠

國立虎尾科技大學/文理學院/生物科技研究所/碩士(2014年)

摘要

蜂王漿已被證明具有免疫調節活性，尤其是在它的蛋白質成分。然而，尚未有研究表明蜂王漿之脂肪酸對免疫系統的影響。蜂王漿之主要脂肪酸為癸烯酸，且已被證實具有抗發炎效果。在本研究中，探討癸烯酸作用於WiDr細胞的細胞存活率及主要發炎激素之分泌。利用不同濃度之癸烯酸作用於WiDr細胞，探討癸烯酸對其細胞之作用。癸烯酸在高劑量 (5 mM) 下對WiDr細胞具有毒性，在低劑量下 (2和3 mM)則不具細胞毒性，並對IL-8，IL-1β，與TNF-α有顯著的抑制效果。並且顯著誘導IL-1受體拮抗劑之分泌。體外試驗結果顯示，在低劑量癸烯酸下，具有顯著的抗發炎效果，可能具有成為免疫調節藥物的潛力。

關鍵字

癸烯酸 ； 抗發炎 ； WiDr細胞 ； 大腸癌細胞 ； 及細胞激素

並列摘要

Royal jelly (RJ) has been shown to possess immunomodulatory activity, especially its protein components. However, almost nothing is known about influence of RJ fatty acids on the immune system. The anti-inflammatory effect of 10-hydroxy-2-decenoic acid (10-HDA), a major fatty acid in RJ was investigated. In this study, we studied the effect of 10-HDA on the cell viability and the release of important inflammatory cytokines from WiDr cell. To investigate the effect of 10-HDA, we treated WiDr human colon cancer cells with various concentration of 10-HDA. The higher dose (5 mM) of 10-HDA has cytotoxic effect on WiDr, it could directly kill the colon cancer cells. A lower dose of 10-HDA (2 and 3 mM), although non-cytotoxic, but it has significant inhibition of the pro-inflammatory cytokines. The cytokines that inhibited are interleukin (IL)-8, IL-1β, and tumor necrosis factor-alpha (TNF-α). It also significantly induces receptor antagonist cytokine (IL-1ra). 10-HDA inhibited the cell growth of WiDr in a dose-dependent manner. These data suggest that a non-toxic concentration of 10-HDA has a significant effect on the in vitro growth of WiDr cells, and may have value as a therapeutic immunomodulatory property.

並列關鍵字

10-hydroxy-2-decenoic acid (10-HDA) ； Anti-inflammatory ； WiDr cells ； Colon cancer cell ； Cytokines

參考文獻

[6] Lercker, G., et al., 1981, Components of royal jelly: I. Identification of the organic acids, Lipids 12:912–919.

[7] Plettner, E., Slessor, K. N.,Winston, M. L., 1998, Biosynthesis of mandibular acids in honeybees (Apis mellifera): de novo synthesis, route of fatty acid hydroxylation and caste selective b-oxidation, Insect Biochem Mol Biol 28:31–42.

[8] Ferioli, F. M., Marcazzan, G. L., Carboni, M. F., 2007, Determination of (E)-10-hydroxy-2-decenoic acid content in pure royal jelly: a comparison between a new CZE method and HPLC, J. Sep. Sci 30(7): 1061-1069.

[9] Antinelli, J.-F., et al., 2003, Evaluation of (E)-10-hydroxy-2-decenoic acid as a freshness parameter for royal jelly, Food Chem. 80,85-89.

[11] Zhou, J., Zhao, J., Yuan, H., et al., 2007, Comparison of UPLC and HPLC for determination of trans-10-hydroxy-2-decenoic acid content in royal jelly by ultrasound-assisted extraction with internal standard, Chromatographia, 66, 185-190.