馬來西亞無螫蜂蜂蜜之抗氧化及抗菌特性分析與研究

Investigations of the Antioxidant and Antibacterial characteristics of the Meliponinae honey from Malaysia

拉莉塔(Lalita Tuksitha)

指導教授 ： 彭及忠

國立虎尾科技大學/文理學院/生物科技系碩士班/碩士(2017年)

摘要

無螫蜂是一群沒有刺針的蜜蜂，主要生長在熱帶和亞熱帶地區，屬於Meliponinae物種，他們可以生產不同類別且富含有抗氧化、抗微生物活性有關之酚類和類黃酮化合物的無螫蜂蜜。因此，本研究探討分析馬來西亞無螫蜂蜜的物理化學性質及抗氧化活性和抗菌活性，其中包括了Genotrigona thoracica，Heterotrigona itama和Heterotrigona erythrogastra，由結果得知，G. thoracica 蜂蜜在類黃酮含量（99.04±5.14 mg/ml）有著最高濃度和還原力（19.05±0.79 ％），然而總酚（17.67±0.75 mg/ml）及還原力（18.10±0.35％），DPPH（47.40±3.18 ％）和FRAP的含量在H. itama蜂蜜 (50.66±5.77 mM of Fe2+ /100 g）均明顯地高於其他蜂蜜。此外，G. thoracica對屬於革蘭氏陽性菌的葡萄球菌 (Staphylococcus xylosus) (2.10±0.10 cm）具有最高的抗菌活性，且還有革蘭陰性菌的綠膿假單胞菌 (Pseudomonas aeruginosa)（1.60±0.10 cm）和副溶血弧菌 (Vibrio parahaemolyticus)（2.03±0.06 cm）。這些結果表明，無螫蜂蜂蜜具有相關量的酚類和類黃酮化合物，可以作為天然抗氧化劑和抗抗菌活性。

關鍵字

無螫蜂蜜 ； Geniotrigona thoracica ； Heterotrigona itama ； Heterotrigona erythrogastra ； 抗氧化活性 ； 抗抗菌活性.

並列摘要

Stingless honey bees form a large group of bees that lack of a sting and are found among Meliponinae species indigenous to various tropical and subtropical regions. They are able to produce “stingless bee honey” that contains divergent categories of phenolic and flavonoid compounds and have been associated with antioxidant and antimicrobial activity. This study examines the physicochemical properties, antioxidant-activity and anti-microbial activity of stingless bee honey from Malaysia that was produced by Geniotrigona thoracica, Heterotrigona itama and Heterotrigona erythrogastra. The results show that G. thoracica honey has the highest concentration of flavonoids (99.04±5.14 mg/ml) and the greatest reducing power (19.05±0.79%), while the total phenolic context (17.67±0.75 mg/ml), reducing power (18.10±0.35 %), DPPH (47.40±3.18 %) and FRAP (50.66±5.77 mM of Fe2+ /100 g) of H. itama honey is significantly higher than those of the other honeys. In addition, G. thoracica honey has the highest antibacterial activity against Staphylococcus xylosus (2.10±0.10 cm), which is Gram-positive bacterium, and against Pseudomonas aeruginosa (1.60±0.10 cm) and Vibrio parahaemolyticus (2.03±0.06 cm), which are Gram-negative bacteria. These results suggest that stingless bee honeys possess useful amounts of phenolic and flavonoid compounds that are able to act as natural anti-oxidants and also have significant anti-microbial activity.

並列關鍵字

stingless bee honey ； Geniotrigona thoracica ； Heterotrigona itama ； Heterotrigona erythrogastra ； anti-oxidant activity ； anti-microbial activity.

參考文獻

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