壹、千里光之成分研究
 貳、苦茶粕抑菌成分之鑑定

千里光(Senecio scandens Buch.-Ham)為台灣產菊科千里光屬之植物，本屬植物全球超過1500種，台灣約有20餘種。台灣產千里光屬植物目前被研究的種類不多，因此本研究選定千里光進行成分研究，希望藉此了解其化學組成。將千里光甲醇粗萃物，經由分配萃取後所得到的生物鹼層、氯仿層及水層，分別利用矽膠管柱層析、Diaion HP-20逆相管柱層析、薄層層析及高效能液相層析進行分離純化，共鑑定出40個化合物，其中有1個為首次發現的新化合物。針對其中含量較多的木脂體類與固醇類化合物進行抗氧化活性試驗，結果發現木脂體類化合物具有不錯的抗氧化活性，值得進一步進行評估。苦茶粕為油茶籽（Camellia oleifera Abel.）榨完油後所留下來的殘渣，已被報導具有抑制立枯絲核菌（Rhizoctonia solani kühn AG-4）生長的效果，但其抑菌之有效成分尚未確定，故本研究希望分析鑑定苦茶粕中所含有之抑菌成分。將苦茶粕甲醇粗萃物，利用抑菌活性導引分離，經由逆相管柱層析及高效能液相層析純化，共純化得到2個黃酮類化合物與1個三萜皂苷混合物，進一步測試其抑菌活性，希望藉此發現真正具有抑菌活性的成分，以開發新的生物防治資源。

關鍵字

千里光；苦茶粕

並列摘要

Senecio scandens Buch. -Ham (Asteraceae) is a Formosan Senecio species. This genus is widely distributed throughout the world and comprises more than 1500 species. There are about 20 species in Taiwan and the researches regarding this species were few. Therefore S. scandens was selected as a target for investigations of its phytochemical diversity. The methanol extracts of whole plants of S. scandens were fractionated into alkaloid, chloroform, and water solubles, successively, and further purified using silica gel column chromatography, reversed-phase Diaion HP-20 column chromatography, thin-layer chromatography, and high performance liquid chromatography, respectively. Forty compounds were characterized from the crude extracts of S. scandens and among them compound (1) was a new compound reported for the first time. Furthermore, the isolated lignans and steroids with more quantities were subjected into the examination for their antioxidant activities by DPPH free radical scavenging assay. The results displayed that some of the isolates exhibited significant antioxidant bioactivity and maybe valuable to be evaluated further. The tea seed pomace is the residue of the seeds of Camellia oleifera after the oil was pressed from the seeds, and it was reported to possess the inhibitory bioactivity of the growth of Rhizoctonia solani kühn AG-4. However, the active principles were remained unknown, so that the present study was aimed to characterize the antifungal constituents in the methanolic extracts of the tea seed pomace. The methanol crude extract of tea seed pomace was purified using reversed-phase column chromatography and high performance liquid chromatography with the aid of bioassay guided fractionation. Totally two flavonoids and one saponin mixture were characterized from the tea seed pomace and were subjected into the examination of the antifungal activity. It could help to determine the active principle and discover new biocontrol sources.