壹、辣木根皮與莖皮之成分研究 貳、芝麻種子成分及其抗氧化活性研究 參、中草藥萃取物抑制白菜炭疽菌活性成分研究

1.Constituents from the Root Bark and Stem Bark of Moringa oleifera 2.Constituents from the Seeds of Sesamum indicum and their Antioxidant Activity 3.Bioactive Constituents from the Chinese Medicine Extracts and their Inhibiting Activity Against Colletotrichum higginsianum

林美琪(Mei-Chi Lin)

指導教授 ： 郭賓崇

國立虎尾科技大學/文理學院/生物科技研究所/碩士(2011年)

摘要

辣木（Moringa oleifera Lam）根皮甲醇粗萃物氯仿層對具有艾黴素（doxorubicin）抗藥性的人類乳腺癌細胞（MCF-7/ADR）及老鼠肺纖維化細胞（MLF）具有明顯的細胞毒殺活性，因此本研究選定辣木進行成分研究，希望藉此了解其化學組成。將辣木根皮與莖皮分別利用甲醇熱迴流萃取，分配萃取後進行分離純化後，從辣木根皮與莖皮甲醇粗萃物分別鑑定出27與11個化合物，其中2個為天然界首次發現的新化合物，純化所得化合物將進一步送測藥理活性，希望藉此開發新的天然抗癌藥物。

芝麻（Sesamum indicum）種子甲醇粗萃物，經由分配萃取與各種層析方法進行分離純化，共鑑定出30個化合物，其中包括2個天然界首次發現的化合物與利用半合成反應所得的木酚素類化合物進行DPPH自由基捕捉、超氧陰離子抑制、亞鐵離子螯合能力及還原力實驗評估其抗氧化活性。結果顯示，除了sesamin及sesamolin等含量較多的木酚素之外，其他芝麻木酚素化合物如 (+)-pinoresinol、(-)-piperitol及(+)-(7S,8'R,8R)-acuminatolide都具有顯著的抗氧化活性，顯示其具有開發成為保健食品的潛力。

在有機蔬菜栽培田易發生由白菜炭疽菌（Colletotrichum higginsianum）引起的白菜炭疽病，因此本研究選用了五倍子、丁香、肉桂、大黃及虎杖等五種中藥材，利用高效能液相層析定量分析其粗萃物及分畫層中指標成分的含量，並進一步測試其粗萃物、分畫層與指標成分對白菜炭疽菌的抑菌活性，希望能藉此發現有效防治病害的成分，進而開發新的植物保護製劑。

關鍵字

辣木 ； 芝麻 ； 白菜炭疽菌

並列摘要

In the preliminary cytotoxic bioassay, the chloroform layer of methanol extracts of M. oleifera demonstrated the significant cytotoxicity toward the doxorubicin resistant human breast adenocarcinoma cell lines (MCF-7/ADR) and rat pulmonary fibrosis cells (MLF). Therefore, the present research is aimed to purify and identify the constituents of M. oleifera. The methanol extracts of root barks and stem barks of M. oleifera were fractionated and further purified to afford 27 and 11 compounds, respectively. Among the isolates two compounds was reported from the natural sources for the first time. These isolates would be subjected into the biological activity examinations to explore new natural anticancer drugs.

The methanol extract of sesame (Sesamum indicum) seeds was fractionated and purified to afford 30 compounds. Among these isolates two were isolated from the natural sources for the first time. To evaluate their in vitro antioxidant potentials, the purified lignans and their derivative prepared by semi-synthesis were examined for the scavenging activities of DPPH free radicals and superoxide anions. Moreover, the capability of chelating ferrous ions and reducing power of these sesame lignans were also tested. The results suggest that, besides the well-known sesamolin and sesamin, the minor sesame lignans (+)-pinoresinol, (–)-piperitol and (+)-(7S,8'R,8R)-acuminatolide are also adequate active ingredients and may be potential sources for the development of healthy foods.

In the organic vegetables cultivation fields, the cabbage anthracnose caused by Colletotrichum higginsianum was a serious problem. Thus in the present study, five different traditional Chinese medicines, including Galla rhois, Eugenia caryophyllata Thunb., Cinnamomum cassia, Polygonum cuspidatum Sieb. et Zucc., and Rheum officinale Baillon were extracted and purified. The contents of indicator compounds in the crude extracts and fractions were analyzed with the aid of high-performance liquid chromatography. Furthermore, the antifungal activities of the crude extracts, fractions, and indicator compounds were examined to explore the active principles against C. higginsianum. From our research results, it is hopeful to discover new botanical pesticides derived from the plant sources.

並列關鍵字

Moringa oleifera ； Sesamum indicum ； Colletotrichum higginsianum

參考文獻

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