This study investigated the effect of different light sources on the bubble culture of photosynthetic bacteria, experiment with three kinds of light-emitting diode red, white, blue, LED light source to simulate different body into different modes of rectangular slot to discuss the distribution of LED light intensity inside the photosynthetic bacteria illumination effects. Rectangular tank influence in different media (air, water, gas and liquid) measured in the study found the illumination light source and optical quantum aqueous medium for the three media article explores the strongest intensity of light, followed by gas-liquid mixed media, and finally is the air medium. Three light illumination and light quantum Comparison from large to small are: white, blue, red, gas-liquid mixed media results slightly lower than the water medium light intensity light intensity, but the gap between the two is very small.