以表面固化酶分解高科技產業廢水中之過氧化氫

A surface-immobilized catalase for H2O2 degradation in high-tech industrial wastewater

陳銥頵(Yi-Jiun Chen)

指導教授 ： 黃志彬

國立交通大學/工學院/環境工程系所/碩士(2019年)

摘要

半導體產業於晶圓清洗與研磨時會產生大量含H2O2廢水，目前實廠多以直接投加過氧化氫酶(catalase,CAT)溶液方式處理含H2O2廢水，然此法易造成CAT過度耗用，大幅增加處理成本。為增加CAT可處理的廢水量
及其重複使用次數，本研究以商用弱酸型陽離子交換樹脂作為載體，以 Ca2+離子為交聯劑將海藻酸鈉批覆於樹脂表面，並同時將CAT包埋於海藻酸鹽與樹脂表面結構中，進行酵素固定化。以表面帶有-COOH官能基的弱酸型陽離子交換樹脂為載體，先將其浸泡於3M CaCl2溶液中，透過離子交換將-COOH上的H+離子置換成Ca2+離子後，再將3g處理後的樹脂投入alginate-CAT混合液中，使海藻酸鹽以錯合方式錯合於樹脂表面，同時將CAT包埋於海藻酸鈉與樹脂間的間隙中，以製備披覆海藻酸鈉固化酶樹脂(以固化酶稱之)材料。結果顯示，3g固化酶於15分鐘內幾近完全地降解 14.71mM H2O2，且重複60次反應後，於相同時間內仍有近99%的降解能力。本研究以樹脂為載體所開發的酵素表面固化技術除了可有效維持酵素反應穩定性，且具材料再生能力，並可大幅提高酵素處理廢水效能。

關鍵字

過氧化氫 ； 過氧化氫酶 ； 酵素固定化 ； 再生

並列摘要

Wastewater from semiconductor industry contains a large amount of hydrogen peroxide (H2O2) which is used in the process of wafer etching and grinding. Direct addition of catalase (CAT) solution is a commonly used method to treat the H2O2 containing water. Although this method is effective, constant loss of CAT with the wastewater flow dramatically increase the cost for the treatment. In order to reduce CAT consumption, this invention immobilized CAT onto a commercial ionic exchange resin with alginate and Ca2+ ions. The resin with –COOH functional groups was firstly treated by CaCl2 solution to replace the H+ ions of the –COOH groups with Ca2+ ions. The treated resin was then put into a CAT-alginate mixture solution to allow the alginate to complex to the adsorbed Ca2+ ions and entrap the CAT within the interstitial space between the alginate and the resin at the same time. The 3 g CAT-immobilized resin beads have been demonstrated to entirely decompose 14.71 mM H2O2 in the 100 mL solution within 15 minutes, and nearly 99% activity of the immobilized CAT still maintained after undergoing 60 cycles of the decomposition. The material could be regenerated by 0.3 M HCl solution after enzyme inativation. These results indicate that the surface-enzyme-immobilization on a commercial resin not only enables the product to withstand high pressure in the filed treatment but also facilitates mass transfer within the substrate to promote the reaction kinetics and retains the enzyme to stabilize the activity for long-lasting water treatment.

並列關鍵字

H2O2 ； catalase ； CAT ； enzyme immobilization ； regeneration

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