The thesis is devoted to develop nanodiamonds which possess single photon emission at room temperature. In order to improve the generation rate of a single photon source, nanodiamonds placed on the gold film which is coated with a dielectric material enhanced spontaneous emission could be reached. Via fluorescence spectroscopy and second-order correlation functions measurements, the single-photon sources could obtain higher emission rate by using this hybrid-system. Theory and simulation results confirmed that plasmon polariton in dielectric layer produce high-density confinement of the electric field. Hence, (N-V)- center with strong electric field can lead to a higher rate of spontaneous emission.