Recently, liquid scintillation detectors are extensively used with particle detectors in many projects such as Dayabay. Mostly in such detectors, photomultiplier tubes (PMTs) are used for the detection of light. The gains of PMTs have closely relation with the calculation for particle energy, so it is crucial for calibrating the gains of PMTs. Such as Dayabay, there are two methods, Dark noise and LED light, to get the gains of PMTs. In this thesis, we discuss the discrepancy in the two methods.