液晶與高分子前驅物複合材料生物感測器

Liquid Crystal‒Prepolymer Composite Biosensor

顏世淳(Shih-Chun Yen)

指導教授 ： 李偉 ； 李孟娟

國立交通大學/光電學院/光電系統研究所/碩士(2020年)

摘要

本論文提出一種基於高分子與液晶複合材料的生物感測手段，可應用於牛血清蛋白之檢測。經過紫外光曝照高分子聚合後，實驗結果顯示吾人得以達到更佳之偵測極限及偵測靈敏度，背後的機制是摻雜低濃度光固化高分子預聚物於液晶中表現出特殊的預傾角調控特性及折射率不匹配導致的光散射。本研究採用高分子/液晶複合系統做為牛血清蛋白偵測手段，搭配介電常數的量測進行牛血清蛋白濃度定量分析，再利用介電常數值換算出液晶等效傾角後，該等效傾角與牛血清蛋白的一對一關係擬合出線性函數來做為定量分析的依據，此偵測手段所達最佳偵測極限為10-10 g/ml。

關鍵字

高分子‒液晶複合材料 ； 生物感測器 ； 牛血清蛋白

並列摘要

We propose to LC–prepolymer (E7–NOA65) as the sensing element for protein detection. After irradiating ultraviolet light to perform the photopolymerization process, our results indicate that the limit of detection can be promoted due to the enhancement of the optical signal, derived from the controlling of the LC’s pretilt angle and light scattering. In this study, the polymer–liquid crystal composite system was used as the detection method of bovine serum protein, combined with the measurement of dielectric constant for quantitative analysis of the concentration of bovine serum protein, and then the dielectric constant value was used to calculate the equivalent tilt angle of the liquid crystal. The one-to-one relationship between tilt angle and the concentration of bovine serum protein is fitted to a linear function as a basis for quantitative analysis. The best detection limit reached by this method is 10-10 g/ml.

並列關鍵字

LC‒prepolymer composite ； biosensor ； bovine serum albumin

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