Anodic aluminum oxide (AAO) thin films have been studied as an alignment layer for liquid crystal (LC) in recent year. Because the ITO thin film can be etched by the anodizing process, the choosing of electrode material under the AAO thin film has become an important subject. In this work, the liquid crystal (LC) cell with the anodic aluminum oxide on ITO glass has been demonstrated. After the ITO film has been modified by O2 plasma, a titanium film as an adhesion layer has been evaporated between ITO film and aluminum film. The liquid crystal molecules was aligned vertically on anodic aluminum oxide film with ITO glass and it can be controlled by applying electric field. The measured response time is 90.8 (ms) and polar anchoring strength is 1.32 x 10-4 (J/m2)