Trichoderma reesei與Aspergillus niger共醣化稻稈及Saccharomyces cerevisiae生產生質酒精之研究

Trichoderma reesei and Aspergillus niger co-saccharification rice straw and production of bioethanol by Saccharomyces cerevisiae

郭以謙(Yi-chin KUO)

指導教授 ： 徐敬衡

國立中央大學/工學院/化學工程與材料工程學系/碩士(2014年)

摘要

摘要
近年來由於能源危機和石油價格高漲，以農業廢棄物做為基質生產生質酒精為研究議題漸受重視，也有許多文獻及研究成果，足以說明生質能源目前占有的地位。
本研究的發酵策略是透過液態單獨培養兩株真菌A.niger及T.reesei分別生產纖維水解酵素，然後以增加接菌量的方式來提升酵素的產量，並且以自行生產的豆渣取代複合式氮源來降低發酵成本，在進入醣化階段時，經過實驗分析醣化之物理條件以及適當的醣化時機後以Mixing的方式混合兩真菌的酵素發酵液進行AT共醣化，在共醣化階段欲發揮AT酵素液水解的極限而在該階段總共添加了兩次稻稈，最後將醣化階段的醣化液應用於Saccharomyces cerevisiae酒精發酵。
由實驗結果顯示，本研究第一階段的AT混合酵素液最大活性為0.511 FPU/mL，而在第二階段可以從3g稻稈醣化出的還原醣累積量為21.25g/L，水解效率達到85.2%，在第三階段酒精發酵的酒精生產量為7.172 g/L，轉化率為76.7%。

關鍵字

Trichoderma reesei ； Aspergillus niger ； Saccharomyces cerevisiae ； 酵素

並列摘要

Abstract
The study is to develop a fermentation strategies by liquid monocultural two strains of fungi A.niger and T.reesei production hydrolysis enzymes, and then to increase the inoculum way to enhance the production of enzymes, and to replace the nitrogen compound source by self-production of bean dregs to reduce the cost of the fermentation process, when entering the saccharification stage,after study the physical conditions and the timing of saccharification,and then by Mixing a way to mix the enzyme fermentation broth of two fungi called AT co-saccharification.
To achieve maximum effect of hydrolysis of AT enzyme solution,at co-saccharification stage the rice straw was added twice the saccharification stage of the hydrolyzate,used in the final stage of ethanol fermentation by Saccharomyces cerevisiae.Experimental results show that, AT mixed enzyme solution of the present study the maximum cellulase activity was found to be 0.511 FPU / mL, at optimum condition and from the saccharification of 3g rice straw in the second stage were obtained the maximum reducing sugar accumulation of 21.25g / L, the hydrolysis efficiency of 85.2 %, the amount of ethanol produced of 7.172 g / L in the final stage, the conversion yield was 76.7%.

並列關鍵字

bioethanol ； cellulase

參考文獻

2. Rashmi Kataria, S.G., Saccharification of Kans grass using enzyme mixture from Trichoderma reesei for bioethanol production. 2011.

3. Aftab Ahamed, P.V., Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor. 2008.

4. Gurpreet Singh Dhillona, b., Harinder Singh Oberoia, Surinder Kaurb,c, and S.K.B. Sunil Bansald, ∗, Value-addition of agricultural wastes for augmented cellulase and xylanase production through solid-state tray fermentation employing mixed-culture of fungi. 2011.

5. Namita Bansal a, R.T.b., Raman Soni c, Sanjeev Kumar Soni a,⇑, Production of cellulases from Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues. 2012.

6. Carmen Sánchez, Lignocellulosic residues: Biodegradation and bioconversion by fungi. 2009.