以雷射光控制膠原蛋白字組成之結構

Light-controlled self-assembly of collagen

蘇堡銓(Pao-chuan Su)

指導教授 ： 陳賜原

國立中央大學/理學院/生物物理研究所/碩士(2014年)

摘要

膠原蛋白代表了胞外基質的主要結構蛋白，闡明其裝配的機制對於理解許多細胞生物學和醫學過程，以及用於組織工程和生物技術的方法，更是學術研究的主軸，但是目前研究方向不外乎為明膠與膠原纖維的大尺度比較，卻只有對於外觀上D-period的多寡證實材料製程上的可信度。

　　然而在微尺度的研究上，不管是用機械力或外加磁場都並未真正有效達成定向纖維的薄膜控制，且內部的交聯結構並未進行探究，也尚未沒有一個確切的模型，近年來發現在雲母片及二氧化鉬可以使低濃度下的膠原長成單片薄膜，對於膠原蛋白在體外的自組成上的有更加一步地探究模型，這也是我這篇論文最主要的軸心，我們利用此基板的特性為出發點，進行對膠原蛋白結構上可能的控制變因，並設計出了用雷射光的受激拉曼效應使局部材料吸共振吸收，導致局部性的材料加熱，並採用光壓達到薄膜上材料的指向穩定控制。

關鍵字

膠原蛋白薄膜 ； 受激拉曼散射 ； 光壓 ； 表面增強拉曼散射 ； 奈米線 ； 光極化

並列摘要

Collagen represents the major structural protein of the extracellular matrix. Elucidating the
mechanism of its assembly is important for understanding many cell biological and medical
processes as well as for tissue engineering and biotechnological approaches.
In this work, conditions for the self-assembly of collagen type I molecules on a supporting
surface were characterized.
By applying deposition dynamics deposition of collagen on a substrate at room temperature
using an appropriate solvent, collagen assembled into ultrathin ( 4nm) highly anisotropic ribbon-like
structures coating the entire support.
We use stimulated Raman scattering system enables absorption of specific functional groups to
allow local resonance absorption, causing local heating of the material, and applying a
ponderomotive force to the collagen fibers, in order to achieve a stable directional control.

並列關鍵字

collagen microribbons ； Stimulated Raman Scattering ； Ponderomotive Force ； Surface Enhanced Raman Scattering (SERS) ； Nanowire

參考文獻

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