玻尿酸/明膠複合微水膠自組裝對誘導式多能幹細胞分化的效應

本研究利用天然高分子玻尿酸與明膠以甲基丙烯酸酐改質，以2-hydroxy-1-(4-(hydroxyethoxy)phenyl)-2-methyl-1-propanone為光起始劑與光罩透過波長365 nm的ultraviolet照射形成微水膠。接著把微水膠放到礦物油中，因親水性與親油性的不同，使微水膠自組裝水膠，提供一個包埋幹細胞的方法。 FTIR顯示改質後的明膠與玻尿酸皆有接上甲基丙烯酸，FTIR在1650~1688 cm-1烯類官能基的波谷有增加。玻尿酸/明膠複合水膠提升玻尿酸的比例在XPS分析中，鈉的波峰強度增加。膨潤度方面隨著濃度的下降而上升，約在20~70倍之間，顯示親水性良好，可以提供幹細胞一個良好的生長環境。水膠在6%時細胞包埋率達80%以上，當濃度的增加包埋率上升。玻尿酸比例高的水膠在H&E染色顯示誘導式多能幹細胞(induced pluripotent stem cell, iPS)與材料有良好的貼附，且維持iPS在未分化態，以NGF進行誘導成神經，有高度的β-III tubulin表現量，說明玻尿酸適合用來包埋幹細胞進行分化培養。

關鍵字

光交聯；玻尿酸；明膠；誘導式多能幹細胞；微水膠；自組裝

並列摘要

In this research, we choose two natural materials: hyaluronic acid (HA) and gelatin, modified with methacrylic anhydride (MA), and a low cyototoxicity photoinitiator, 1-hydroxy-1-(4-(hydroxyethoxy)phenyl)-2- methyl-1-propanone, to synthesis hybrid microhydrogels by a photomask and exposing ultraviolet (365 nm). Microhydrogels are transfered to mineral oil to self-assembly that provides one way to encapsulate stem cells. After modifying, hyaluronic acid and gelatin become methacrylated hyaluronan (HAMA)and methacrlated gelatin (GelMA). The FTIR spectra shows that the peak at 1650~1688 cm-1 for C=C increases. It means that both of HA and gelatin reacted with MA and have acrylate group. Because that used HA is sodium type, as the percentage of HAMA in hydrogel increases, the peak at 1070 eV for Na of XPS indreases. As gel concentration decreases, swelling ratio of hydrogel increases, and the range of swelling ratio is about 20 to 70 time. It shows that hydrogel made a good environment because of good hydrophilic properties. At high percentage of HAMA, induced pluripotent stem cells (iPS) have good adhesion to material, and are maintained in undifferentiated state. As iPS cell-laden hyrogel was treated with nerve growth factor, iPS expressed more β-III tubulin. It shows that HAMA is a fine material to encapsulate stem cell to differentiate.

並列關鍵字

photocrosslink ； HA ； gelatin ； iPS ； microhydrogel ； self-assembly

參考文獻

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玻尿酸/明膠複合微水膠自組裝對誘導式多能幹細胞分化的效應

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