黃原膠/玻尿酸/石墨烯複合式薄膜作為傷口癒合敷料之研究

黃原膠(Xn)是一種由黃單胞菌 (Xanthomonas campestris)分泌的一種多醣體，常用於食品添加物。玻尿酸 (HA)是一種陰電性多醣體，分佈於結締組織、上皮和神經組織。石墨烯（Graphene、GO）是一種由碳原子組成六角型呈蜂巢晶格的平面薄膜，只有一個碳原子厚度的二維材料。本研究為了發展導熱式的傷口敷料，製備Xn/HA/GO(0.03g)薄膜，利用1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)作為交聯劑，製備具有含膠量87.4%、含水量80.0%和應力強度5.39 Kgf/mm2的薄膜。為了評估熱效應對傷口癒合的影響。Xn/HA/GO(0.03g)薄膜被植入老鼠皮下的傷口，每天以805 nm的雷射光照射20分鐘，持續7天。實驗結果發現，Xn/HA/GO(0.03g)不會引起傷口的發炎反應，而且經過熱療程序的傷口，不論是傷口癒合百分比或是膠原蛋白的合成量都高於未經熱療處理的傷口癒合。本研究證實導熱式的Xn/HA/GO(0.03g)傷口敷料，有助於傷口的癒合。

關鍵字

黃原膠；玻尿酸；石墨烯；導熱式傷口敷料

並列摘要

Xanthan gum (Xn) is a polysaccharide secreted by the bacterium Xanthomonas campestris, used as a food additive. Hyaluronic acid (HA), is an anionic, distributed widely throughout connective, epithelial, and neural tissues. Graphene (GO) is an allotrope of carbon in the form of a two-dimensional, atomic-scale, honey-comb lattice in which one atom forms each vertex. To develop a thermally conductive wound dressing, in this study, we prepared Xn/HA/GO(0.03g) film with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) in 80% ethanol to obtain a cross-linked film with 87.4% gel content, 80.0% water content and 5.39 Kgf/mm2 tensile strength. To assess the effect of thermal therapy for wound healing. Xn/HA/GO(0.03g) film implanted into rat subcutaneous tissue and were irradiated by a 805 nm laser 20 mins each day for 7days , the Xn/HA/GO(0.03g) film not caused inflammation during the period. The results indicate that the effects of Xn/HA/GO(0.03g) film with thermal therapy on wound healing, wound size reduction (%) and collagen content are higher than those without thermal therapy.