雙面光柵波導生物感測晶片之綠膿桿菌抗藥蛋白偵測

Double-sided waveguide grating biosensor of Pseudomonas aeruginosa resistant protein detection

邱妍芯(YAN-SIN CIOU)

指導教授 ： 謝文馨

國立中正大學/工學院/機械工程學系/碩士(2016年)

摘要

本研究目的發展一個低成本、可即時偵測、高靈敏度、可量產化之雙面光柵波導生物感測器生物檢測系統，並應用於發展抗藥性細菌之快篩平台。 製程上利用光柵結構模仁並配合射出成型製作晶片，透過濺鍍機在晶片表面沉積二氧化鈦波導層形成雙面光柵波導結構。在進行抗藥蛋白檢測前，本研究先進行DNP/ anti-DNP之生化檢測，其偵測極限為1.7x〖10〗^(-7) g/ml。在進行抗藥蛋白檢測時，我們在雙面光柵波導生物感測器表面成功的修飾上綠膿桿菌抗藥蛋白抗體E3，然可能由於波導表面漸逝波太短、抗體鍵結太多，所以感測不到OprD抗藥蛋白。

關鍵字

綠膿桿菌 ； 抗藥蛋白 ； 雙面光柵波導生物感測晶片 ； 免疫

並列摘要

This study was to develop a double-sided waveguide grating biosensor, with features of low-cost, real-time detection, high sensitivity and mass production, which was used as the rapid screening platform for the detection of drug-resistant bacteria. The biosensor was prepared with an injected COC grating structure of which surface was sputtered with a layer of TiO2 waveguide. On the detection of DNP / anti-DNP reaction, the detection limit was 1.7x10^-7 g/ml. In the detection of Pseudomonas aeruginosa resistant proteins, we have succeeded in modifying the E3 antibody on the waveguide. The detection of Pseudomonas aeruginosa resistant proteins was not achieved, probably due to the fact that the evanescent wave was too short to cover the modified antibodies on the waveguide surface.

並列關鍵字

Pseudomonas aeruginosa ； resistant protein ； double-sided waveguide grating biosensor ； immunoassay

參考文獻

[1] 林梅芳 and M.-F. Lin, "綠膿桿菌感染之治療-文獻回顧."

[2] A. Farra, S. Islam, A. Strålfors, M. Sörberg, and B. Wretlind, "Role of outer membrane protein OprD and penicillin-binding proteins in resistance of Pseudomonas aeruginosa to imipenem and meropenem," International journal of antimicrobial agents, vol. 31, pp. 427-433, 2008.

[3] S. D. Mazumdar, B. Barlen, T. Kramer, and M. Keusgen, "A rapid serological assay for prediction of Salmonella infection status in slaughter pigs using surface plasmon resonance," Journal of microbiological methods, vol. 75, pp. 545-550, 2008.

[4] X. Wei and S. M. Weiss, "Guided mode biosensor based on grating coupled porous silicon waveguide," Optics express, vol. 19, pp. 11330-11339, 2011.

[5] F. El Garch, P. Bogaerts, C. Bebrone, M. Galleni, and Y. Glupczynski, "OXA-198, an acquired carbapenem-hydrolyzing class D β-lactamase from Pseudomonas aeruginosa," Antimicrobial agents and chemotherapy, vol. 55, pp. 4828-4833, 2011.