開發離子液體靜相於超臨界流體層析並應用於食品分析

The Development of Ionic Liquid Stationary Phases of Supercritical Fluid Chromatography for Food Analysis

李怡萱(YI-SYUAN LI)

指導教授 ： 魏國佐

國立中正大學/理學院/化學暨生物化學系/碩士(2016年)

摘要

在綠色化學成為重要的議題下，層析所用的揮發性溶劑也成為綠色分析化學探討的議題，而超臨界流體層析 (Supercritical fluid chromatography, SFC) 使用無毒的二氧化碳作為主要動相，再添加少許的有機溶劑做輔助，因此大大減少了有機溶劑的使用量，是一個具有綠色化學概念的層析技術。然而目前SFC所使用的管柱都採用傳統液相層析的管柱，這些管柱無法呈現SFC的特性，導致影響SFC的性能，因而降低了SFC的普遍性。目前為止除了2-EP外，極少為SFC性能設計的管柱，我們的研究團隊首先提出以離子液體填充靜相為發揮SFC性能設計的管柱，因為離子液體靜相能夠同時提供出多重的作用力，能有效分離不同官能基的分析物，將大大增進SFC的實用性。
本研究第一部份為篩選實驗室自製離子液體管柱，並探討其分離塑化劑與木酚素的效能。本研究第二部分將離子液體鍵結AgTCM上，同時利用LSER比較一系列管柱(MPS、MPS-Ag、MPS-Ag-mIm-NH2)作用力的差別，並探討MPS-Ag、MPS-Ag-mIm-NH2對於多苯環與甘油脂類化合物的分離效果。

關鍵字

超臨界流體層析 ； 離子液體 ； 塑化劑 ； 木酚素

並列摘要

As green chemistry has become an important subject recently, the use of organic solvents in chromatography becomes an issue of green separations. Supercritical fluid chromatography uses a nontoxic medium, supercritical carbon dioxide, along with small amount of organic solvents as the co-solvents for the separation. Therefore, the amount of organic solvent used in SFC is far less than that of conventional high performance liquid chromatography (HPLC). Therefore, SFC is regarded as a green analytical technique. However, most SFC separations directly adapt traditional HPLC columns that are not designed for SFC and lead to a poor separation performance, as compared with that of HPLC. Thereby SFC does not meet the expectation and becomes a less favor chromatographic technique since its invention. The use of right column is one of the main tasks to improve SFC performance. So far there are very few special design columns for SFC. Our group is the first one to explore the use of ionic liquid functionalized stationary phases as SFC columns. Since the ionic liquid stationary phases can provide several kinds of molecular interactions. With the feature of multimode interactions from ionic liquid columns, it is feasible that a single column can effectively separate the mixture of different functional compounds.
In this study, we screened previously developed ionic liquid stationary phases to separate plasticizer and lignans. With the screened stationary phases, the separation conditions were optimized for the determination of plasticizer and lignans in real samples. Then we explored a new stationary phase which has silver and ionic liquid bonded onto silica gel and use the linear solvation energy relationship (LSER) model to characterize the molecular interactions of the column. Afterward, these columns were investigated to separate PAHs and glycerols.

並列關鍵字

SFC ； ionic liquid ； plasticizer ； lignan

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