波導模態共振結合粒子電漿共振用來提升化學與生物化學分子感測之感測器

Guided mode resonance enhanced chemical and biochemical sensing by particle plasmon resonance sensor

林伯勳(PO-SYUN LIN)

指導教授 ： 周禮君

國立中正大學/理學院/化學暨生物化學系/碩士(2016年)

摘要

在本研究中，我們的感測平台是藉由波導模態共振(GMR)效應來增強粒子電漿共振(PPR)的效應，結合了這兩種效應的原理開發出一款新型的生物感測器。感測晶片的構造是使用波導模態共振光柵晶片並在光柵波導層表面分別修飾上球形以及棒形金奈米粒子。在本研究中，我們藉由改變外在環境的折射率，探討不同的入射角度下，GMR的效應結合PPR的效應會對整體的感測靈敏度帶來什麼樣的趨勢與變化，並將平台最佳化的感測條件建立起來。經由實驗測試，GMR的效應結合球形金奈米粒子的PPR效應，利用532 nm雷射的即時偵測系統進行環境折射率變化的感測靈敏度測試，當入射角為20度，GMR共振模態在536 nm時，靈敏度可達3.0 RIU-1左右。與純粹只有球形金奈米粒子的PPR相比時大約增強了6倍左右。另一方面，GMR的效應結合棒形金奈米粒子的PPR效應，利用785 nm雷射的即時偵測系統進行環境折射率變化的感測靈敏度測試，當入射角為8.5度，GMR共振模態在779 nm時，靈敏度可達21.1 RIU-1左右。與純粹只有棒形金奈米粒子的PPR相比時大約增強了40倍左右。
在生化感測方面，當入射角為20度，GMR共振模態在536 nm時，GMR結合球形金奈米粒子的粒子電漿共振效應，在Anti-DNP的檢測上偵測極限(LOD)為7.75×10-9 g/mL (5.17×10-11 M)。與純粹只有球形金奈米粒子時的LOD，1.36×10-7 g/mL (9.07×10-9 M)，增強了約18倍。再與只有GMR效應的光柵晶片相比時，其LOD為7.81×10-8 g/mL (5.27×10-10 M)，增強了10.2倍。同時，初步實驗也証實了金棒結合GMR是具有生化感測能力的。

關鍵字

波導模態共振 ； 粒子電漿共振 ； 生物感測器 ； 金球奈米粒子 ； 金棒奈米粒子

並列摘要

In this study, a novel biosensing platform based on enhancement of particle plasmon resonance (PPR) effect by guided mode resonance (GMR) is demonstrated. The sensing chip is constructed by immobilization of gold nanospheres (AuNSs) or gold nanorods (AuNRs) on a waveguide grating. Since the angle of incident light is crucial for GMR measurement, we explore the relationship between angle of incident light and refractive index (RI) sensitivity of the sensor. Results show that when the incident angle is 20 degree and the GMR resonance wavelength is 536 nm, the GMR-PPR sensor using AuNSs has achieved a RI sensitivity of 3.0 RIU-1 at an excitation wavelength of 532 nm. Such a RI sensitivity shows that the combination of PPR and GMR effects produces a considerable enhancement of 6 times compared to that by the PPR effect alone. Furthermore, when the incident angle is 8.5 degree and the GMR resonance wavelength is 779 nm, the GMR-PPR sensor using AuNRs has achieved a RI sensitivity of 21.1 RIU-1 at an excitation wavelength of 785 nm, which has an enhancement of about 40 times compared to that by the PPR effect alone.
In biosensing, when the incident angle is 20 degree and the GMR resonance wavelength is 536 nm , the GMR-PPR sensor using AuNSs has achieved a limit of detection (LOD) of 7.75×10-9 g/mL (5.17×10-11 M) for Anti-DNP, while the LOD by using the PPR effect of AuNSs alone is 1.36×10-7 g/mL (9.07×10-9 M). Without the PPR effect, the GMR sensor achieves a LOD of 7.81×10-8 g/mL (5.27×10-10 M) for Anti-DNP. Such LODs for Anti-DNP show that when the incident angle is 20 degree and the GMR resonance wavelength is 536 nm, the GMR-PPR sensor using AuNSs produces a considerable enhancement of 18 times compared to that by the PPR effect alone. In addition, the GMR-PPR sensor using AuNSs has a LOD 10.2 times lower than that by the GMR effect alone.

並列關鍵字

guided mode resonance ； particle plasmon resonance ； biosensor ； gold nanosphere ； gold nanorod

參考文獻

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