豬IL-18應用於日本腦炎DNA疫苗之佐劑活性

介白素-18 (Interleukin-18, IL-18) 可促使Th1細胞釋放大量干擾素-γ (Interferon-γ, IFN-γ)，抑制IL-10的產生，具有增強Th1之後天性免疫反應的功效。本實驗以中國倉鼠卵母細胞 (Chinese hamster ovary cells, CHO cells) 分析豬IL-18之真核表現載體的轉染效率，並以4週齡無特定病原 (Specific pathogen free, SPF) 豬隻檢測其作為日本腦炎 (Japanese encephalitis virus, JEV) DNA疫苗之佐劑效益。結果顯示以豬IL-18真核表現載體為佐劑的豬隻之脾臟細胞IL-12及IFN-γ的生成量高於只施打JEV DNA疫苗的豬隻，且其IFN-α以及類鐸蛋白受體 (Toll-like receptor, TLR) 3、7、8的基因表現量高於其他免疫組。因此，真核表現豬IL-18載體具有調節免疫豬隻之佐劑活性，可有效促進其疫苗功效。

關鍵字

介白素-18 ；佐劑； DNA疫苗；豬

並列摘要

Interleukin-18 (IL-18) can enhance the amounts of IFN-γ secreted by T cells and decreases the production of IL-10, and thus reinforces the adpative immunity reaction. This experiment uses Chinese hamster ovary cells (CHO cells) to analyze the transfection efficiency of eukaryotic expression of porcine IL-18 plasmid, and uses the 4 weeks old specific-pathogen free (SPF) pigs to test it and take it as the adjuvant benefit of Japanese encephalitis virus (JEV) DNA vaccine. The result demonstrates that the growing amounts of the splenocytes IL-12 and IFN-γ from the animals injected with IL-18 adjuvant are higher than the pigs that are only injected with JEV DNA vaccine. Moreover, the gene expression of IFN-α and TLR3, 7 and 8 increase yield in the animals injected with IL-18 adjuvant. Therefore, eukaryotic expression of porcine IL-18 plasmid owns the ability to adjust the immune effect of the JEV DNA vaccine that is injected at the same time, which can effectively improve the function of the vaccine.

並列關鍵字

Interleukin-18 ； Adjuvant ； DNA vaccine ； Pig

參考文獻

Agorio, C., Schreiber, F., Sheppard, M., Mastroeni, P., Fernandez, M., Martinez, M.A., and J.A. Chabalgoity. (2007) Live attenuated Salmonella as a vector for oral cytokine gene therapy in melanoma. J Gene Med 9:416-23.

Alexopoulou, L., Holt, AC., Medzhitov, R., and R.A. Flavell. (2001) Recognition of double-stranded RNA and activation of NF-κB by Toll-like receptor 3. Nature 413:732-738.

Anders, H.J., Banas, B., and D.S. Ndorff. (2004) Signaling Danger: Toll-Like Receptors and their Potential Roles in Kidney Disease. J Am Soc Nephrol 15:854-867.

Bach, E.A., Aguet, M., and R.D. Schreiber. (1997) The IFN-gamma receptor: A paradigm for cytokine receptor signaling. Annual Review of Immunology 15:563-591.

Baggiolini, M., Waltz, A., and S.L. Kunkel. (1989) Neutrophil-activation peptide-1/interleukin-8, a novel cytokine that activates neutrophils. J Clin Invest 84:1045-1049.

國際替代計量

豬IL-18應用於日本腦炎DNA疫苗之佐劑活性

全文下載

主題瀏覽