非接觸加熱式液滴型DNA複製系統

Non-contact heating droplet DNA amplification system

謝奕祥(I-Hsiang Hsieh)

指導教授 ： 陳志堅

國立屏東科技大學/工學院/生物機電工程系所/碩士(2013年)

https://doi.org/10.6346/NPUST.2013.00174

摘要

本論文將一台結合伺服馬達的機電定位模組，上方架設鹵素燈管和鼓風扇整合的加熱冷卻系統，自製壓克力反應槽放置於機電定位模組平台上，調整馬達參數控制平台移動，藉由平台在加熱冷卻系統下往復移動，使反應槽內樣本產生高低溫變化，多次往復移動可產生多次熱循環，在生化實驗中有種複製DNA的PCR（polymerase chain reaction）技術，使用溫控器重複改變樣本三種溫度，執行多次熱循環反應，本研究目的為製作一台DNA複製系統，調整機電定位模組上平台往復移動次數代表改變熱循環次數，探討如何用一組加熱冷卻系統搭配平台移動可使反應槽內樣本產生三個不同溫度變化，將燈管照射範圍定義為加熱區，風扇風量影響範圍視為冷卻區，當樣本需要升溫時移動至加熱區內，降溫時在冷卻區內移動，需要溫度維持時移動至受溫度影響較小位置，調整平台以不同速度移動以及在某些區域內短暫停留使反應槽內樣本溫度符合PCR溫度條件，另外為縮短執行PCR時間，本系統採用燈管輻射傳熱方法，並以微量樣本（5~10μl）進行熱循環反應，節省1.5小時反應時間。

關鍵字

非接觸加熱 ； 往復式PCR ； 液滴型樣本 ； 燈管 ； 熱循環

並列摘要

In this paper we develop a DNA amplification system that is combine the DC servo motor, halogen lamp, blower fan and homemade acrylic chamber. Chamber placed on the motor platform, the top set temperature adjustment system consisted of lamp and fan, a sample of the chamber to change in temperature by platform reciprocated, that execution times can produce thermal cycles. A kind of DNA replication technology called PCR in biochemical experiments. This study aimed to produce DNA amplification system. We investigate how to use a set of temperature adjustment system with moving platform can produce three different temperatures that sample of the chamber. The lamp irradiation range is defined as the heating zone, the scope of the fan air flow as the cooling zone. Move the chamber to heating zone when the sample needs to be heated, moving cooling zone during cool down. Adjustment platform moves at different velocities, the sample temperature can be achieved PCR conditions. In order to reduce PCR experiments time, the system uses the 10μl samples for thermal cycling reaction, 1.5 hours reaction time saving.

並列關鍵字

non-contact heating ； Oscillatory PCR ； droplet-based ； lamp ； thermal cycler

參考文獻

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