香蕉花苞片及雄性花萃取物之抗氧化及肝細胞保護作用

Antioxidant and liver cell protective activities of solvent extracts from banana bracts and male flowers

曾國香(Guo-Hsiang Tseng)

指導教授 ： 吳明昌

國立屏東科技大學/農學院/食品科學系所/碩士(2014年)

https://doi.org/10.6346/NPUST.2014.00017

摘要

香蕉Musa spp. (AAA)是多年生草本植物蕉樹的果實，是全世界最普遍的其中一種水果，在全世界都有相當大的產量，據文獻指出香蕉具有豐富的膳食纖維、鉀及鎂等許多對人體有益的物質，也可增加免疫力、抗氧化、抗癌的能力並促進心血管健康、降血壓甚至是對治療憂鬱症也有所作用。而香蕉在結果後，蕉農會為了品質及營養而將香蕉花切除，在其他國家會將其入菜或做成產品，但在台灣，每年所生產的大量香蕉花都被當成農業廢棄物丟棄；而在過往文獻中香蕉有抗炎、保肝的功能，但對於香蕉花尤其是香蕉雄花及香蕉花苞片的著墨並不多。因此，本研究是以在種植香蕉時所產生的廢棄物-香蕉雄花及香蕉花苞片作為原料，利用甲醇、乙醇、蒸餾水三種溶液來行萃取再測定其總酚、總花青素、總類黃酮及其抗氧化能力，並探討香蕉雄花及香蕉花苞片是否會對FL83B細胞具有損傷作用。結果顯示，以甲醇所萃取香蕉雄花的總酚、總類黃酮的含量最高，乙醇萃取香蕉花苞片則是總花青素含量最高；而DPPH自由基清除力及TEAC等抗氧化能力分析也是甲醇萃取香蕉雄花的抗氧化能力最好。正常小鼠肝臟細胞(FL83B)毒性試驗中發現樣品可幫助細胞生長的更好。而在酒精誘導肝臟損傷之保護試驗中甲醇萃取香蕉雄花溶液在濃度400μg/mL即有顯著的保護正常小鼠肝臟細胞的效果其存活率可提高至92.74%，在水萃取香蕉雄花、甲醇萃取香蕉花苞片及水萃香蕉花苞片中則是在濃度800μg/mL有顯著效果，分別是89.39%、91.17%及85.75%。由此研究結果表示，香蕉廢棄物之香蕉雄花及香蕉花苞片具有相當的潛力可開發作為一種良好的抗氧化劑或是酒精性損傷的預防保健食品，並可以此來增加農產品的經濟價值且還可減少廢棄物的產生。

關鍵字

香蕉雄花 ； 香蕉花苞片 ； DPPH ； 抗氧化能力 ； FL83B小鼠肝臟細胞 ； 護肝

並列摘要

Banana Musa spp. (AAA), a member of perennial herb, is the fruit of banana trees. It is one of the most common fruit all over the world due to its high production. According to the literature that the banana has a rich dietary fiber, potassium, magnesium and many other substances beneficial to humans. The banana also increases capacities of immunity, anti-oxidation and anti-cancer, improves cardiovascular health, lowers blood pressure and even affects the treatment of depression. Banana farmers remove the banana flower after banana tree bears fruits in order to control the qualities and nutrition of the banana fruits. Banana flower can be cooked as dishes or made into many other food production; however, tons of banana flower is treated as agricultural waste and dumped in Taiwan every year. In previous studies, banana fruits have showed the capacities of anti-inflammatory and hepatoprotective, but studies related to banana flowers, especially banana buds and bracts, are less than fruits. Therefore, this study was going to analyze total phenol, total anthocyanins, total flavonoids and antioxidant capacity of three solvents extract of banana buds and bracts, which are treated as agricultural wastes while the banana fruits are harvested. Moreover, this study analyzed banana buds and bracts protective ability on injured FL83B liver cells. The results showed that the methanol extraction of banana buds has the maximum contents of total phenol and total flavonoids while the ethanol extraction of banana bracts has the maximum contents of total anthocyanins. Moreover, the methanol extraction of banana buds also has the better antioxidant capacity via DPPH radical scavenging, TEAC and other antioxidant capacity analysis. Toxicity test of mice normal liver cells (FL83B) found that the samples can contribute a better cell growth. And in protection test of the alcohol-induced liver injury, 400μg/mL concentration of methanol extraction of banana buds has significant protection to the mice normal liver cells and their survival rate is up to 92.74%. Besides, 800μg/mL concentration of water extraction of banana buds, methanol extraction of banana bracts and water extraction of banana bracts have obvious results, their rates are up to 89.39%, 91.17% and 85.75% respectively. In conclusion, the banana agricultural waste, such as banana buds and bracts, has considerable potential to be developed as a wonderful antioxidant or a health care food to prevent alcohol injury. Moreover, the new usage of the waste can increase the economic value of agricultural products and also reduce waste.

並列關鍵字

male flowers ； banana bracts ； DPPH ； antioxidant ； FL83B ； hepatoprotective

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