青花菜不同部位之抗氧化力評估

Evaluation of antioxidant activity of various parts of broccoli

黎青寧(Le Thanh Ninh)

指導教授 ： 謝寶全

國立屏東科技大學/國際學院/食品科學國際碩士學位學程/碩士(2015年)

https://doi.org/10.6346/NPUST.2015.00160

摘要

青花菜是一種具有高抗氧化活性的蔬菜。抗氧化劑可以清除自由基並保護人體不受氧化傷害，而氧化壓力是造成心臟、心血管及慢性疾病還有各種形式癌症的主要原因，本研究的主要目的是調查青花菜不同部位的抗氧化活性。取青花菜小花、葉、種子和嫩芽（分3、5、7、10和12天）四種樣品，乾燥後再經由不同濃度的乙醇和熱水萃取，再使用多種分析（ABTS、DPPH、還原力測試）來測定其抗氧化活性。本研究還測定了總酚、總類黃酮及維生素C的含量。之後，將四組樣品的萃取液進行細胞試驗包含肝癌細胞（HepG2細胞）、人類肺癌細胞（A549）和小鼠肝臟正常細胞（FL83B）之MTT試驗。結果顯示，在抗氧化劑的清除力中，利用80% 濃度的乙醇是具有最高能力的萃取溶劑。而在四組樣品中青花菜嫩芽有最高的抗氧化活性，不論是在DPPH（91％清除力、ABTS（6909.5 µmol TE /100g）及還原力測試，也具有較高量的酚類、類黃酮和維生素C含量，其含量分別為2926.6（GAE），730（CE）和378.3（AA）mg/100g。在不同濃度（50 - 500 µg/mL）的嫩芽和種子萃取物中，顯示出樣品可抑制腫瘤細胞的增殖活性（40-50％ cell viability）和幫助正常細胞生長的能力（約120％ cell viability）。不同樣品的萃取物其IC50值約在100 - 400µg/mL間。這項研究可能對消費者的食品選擇有顯著的影響，如再進行深入的研究後，可對未來相關的商業產品提供參考資訊。

關鍵字

青花菜 ； 抗氧化活性 ； 增殖 ； 腫瘤細胞

並列摘要

Broccoli (Brassica oleracea. L) is a potential vegetable with high antioxidant activity. Antioxidants can scavenge free radicals and protect the human body from oxidative stress, which is the main cause of heart, cardiovascular and chronic diseases, and various forms of cancer. Therefore, the main objective of the study is to investigate antioxidant activity of broccoli. Broccoli was used as a testing material and sampled in four groups, including floret, leave, seed and sprout (3, 5, 7, 10 and 12 days). After drying and extraction by different concentrations of ethanol and hot water, the antioxidant activity of four groups were measured using diverse analyses including 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS); 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and reducing power assays. The study also estimated total phenolics, total flavonoids and vitamin C contents in samples. Thereafter, the effect of broccoli extracts on proliferation of cell lines was determined by MTT test in liver cancer cell (HepG2), lung cancer cell (A549) and liver normal cell (FL83B). The results indicated that 80% ethanol was the solvent with highest ability in antioxidant scavenging assays. In four groups of sample, broccoli sprout extracted by 80% ethanol had the highest antioxidant activity analyzed by DPPH (91% scavenging), ABTS (6909.5 µmol TE/100g) and reducing power (3.91 absorbance at 700nm) assays; and contained higher amounts of phenolic, flavonoid and vitamin C at 2926.6 (GAE), 730 (CE), and 378.3 (AA) mg/100g respectively. Sprout and seed extracts showed the highest anti-proliferative activity of tumor cells (40-50% cell viability) and the highest proliferative activity of normal cell (around 120% cell viability) in different concentrations (50 – 500 µg/mL). The IC50 values of different sample extracts were from approximately 100 - 400 µg/mL. The study might have a significant impact on consumers’ food selections and provide scientific information for in-depth research about commercial products.

並列關鍵字

broccoli ； antioxidant activity ； proliferation ； tumor cell

參考文獻

Abdulah, R., A. Faried, K. Kobayashi, C. Yamazaki, E. W. Suradji, K. Ito, K. Suzuki, M. Murakami, H. Kuwano, and H. Koyama. 2009. Selenium enrichment of broccoli sprout extract increases chemosensitivity and apoptosis of LNCaP prostate cancer cells. BMC Cancer 9 (1):414-425.

Aires, A., C. Fernandes, R. Carvalho, R. N. Bennett, M. J. Saavedra, and E. A. Rosa. 2011. Seasonal effects on bioactive compounds and antioxidant capacity of six economically important brassica vegetables. Molecules 16 (8):6816-6832.

Akanitapichat, P., K. Phraibung, K. Nuchklang, and S. Prompitakkul. 2010. Antioxidant and hepatoprotective activities of five eggplant varieties. Food and Chemical Toxicology 48 (10):3017-3021.

Akhlaghi, M., and B. Bandy. 2010. Dietary broccoli sprouts protect against myocardial oxidative damage and cell death during ischemia-reperfusion. Plant Foods for Human Nutrition 65 (3):193-199.

Alam, M. N., N. J. Bristi, and M. Rafiquzzaman. 2013. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal 21 (2):143-152.