應用反轉錄聚合酶鏈鎖反應技術檢測牛隻及豬隻輪狀病毒、諾羅病毒及其親緣關係

Detection of Rotavirus and Norovirus from Swine and Bovine Using RT-PCR and Phylogenetic Analysis

英元(Dewi Murni)

指導教授 ： 廖明輝

國立屏東科技大學/農學院/獸醫學系所/碩士(2016年)

摘要

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關鍵字

輪狀病毒 ； 諾羅病毒 ； 反轉錄聚合酶鏈鎖反應 ； 親緣關係

並列摘要

Rotavirus and norovirus are the primary cause of viral gastroenteritis in humans and animals worldwide. Several studies on humans and animals have shown that rotavirus and norovirus in swine have provided evidence for interspecies transmission. The aim of this study was for study about morbidity rate in swine and bovine in Taiwan, transmission of the viruses to human, and the relationship with the other strains in Taiwan or other countries . This study examined 1,080 (swine) and 720 (bovine) fecal samples collected from 4 regions in Taiwan during January to December 2015. RT-PCR utilizing the primers specific for VP6 gene detected rotavirus with positive reactions 21 (1.9%) in swine and 4 (0.5%) in bovine fecal samples, GIISK gene specific primer detected norovirus with 2 positive reactions (0.2%) in swine and CBECU gene specific primer 3 (0.4%) in bovine. Based on the nucleotide sequences and phylogenetic analysis indicated that 7 of 9 wild strains from swine rotavirus had 84.2 - 93.9% identity relations with those both in human and swine. The wild strains from swine norovirus, bovine rotavirus, and bovine norovirus had 92.5 - 94.5% identity relations with other countries. The wild strains of swine rotavirus and norovirus had 88.3 - 99.4% identities relation with strains from Taiwan, whereas the wild strain from bovine rotavirus and norovirus had lower identity relations (56%) with strains from Taiwan. These findings indicated that the wild strains of swine rotavirus may broadly spread and circulate among farms in Taiwan and contribute to interspecies transmission. Wild strains from both swine and bovine had possibility to cross species traffic among countries. The results can provide reference for control and prevention of disease in veterinary epidemiology.

並列關鍵字

Rotavirus ； Norovirus ； RT-PCR ； Phylogenetic Analysis

參考文獻

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