富血小板纖維蛋白(Platelet-rich fibrin ,PRF)是將血漿離心後取得,富含多種傷口癒合相關的蛋白與生長因子的生物材料。幹細胞(stem cells)是生物體內尚未分化的細胞,具有潛力可分化、增生成各種類型細胞。自1986年在眼角膜與鞏膜交界處(limbus)發現眼角膜幹細胞(corneal stem cells)的存在後,角膜的幹細胞再生治療相關研究便開始大幅增加。在本實驗室的前期研究中,發現PRF有促進幹細胞移動、增生、分化、血管新生、膠原蛋白合成與再生新組織的效果。 在本試驗中,以NaOH破壞兔子的眼角膜製造模擬損傷。被強鹼破壞的角膜,外觀上變的不透明;從切片可看到角膜的Epithelium與Stroma受到破壞。接著,從兔子心臟採血、離心取得PRF萃取液。接著,從兔子的腹部手術切開取得脂肪,純化脂肪幹細胞(adipose-derived stem cells, ADSCs)。以ADSCs搭配PRF萃取液,治療損傷24小時後之眼角膜,連續14天。
Platelet-rich fibrin (PRF), a biologic material isolated from centrifugal blood contains many cytokines and growth factor. Stem cells is undifferentiated cell which has potential to differentiate into different cell types. In 1986, Schermer found corneal stem cells in the limbus and the reports of stem cells reconstruct on cornea was increased. In our studies, we found PRF that can help stem cells express migration, proliferation, differentiation, angiogenesis, collagen synthesis and even regeneration of new tissue. In this study, cornea damage model were induced by immersion in NaOH. Cornea becomes nontransparent. The cornea’s epithelium and stroma were measured with microscope. Then, PRF was isolated from blood by cardiac puncture. The adipose-derived stem cells (ADSCs) was obtained from rabbit’s visceral fat. The damaged corneas were reconstructed with ADMSCs and PRF extract during 14 days treatment.