以動物實驗分析南瓜果實乙酸乙酯萃取物的降血糖效果

Study on the hypoglycemic effect of the ethyl acetate extract of Curcubita moschata fruit in animal models

張智翔(Chih-Shiang Chang)

指導教授 ： 鄭雪玲

國立屏東科技大學/農學院/生物科技研究所/碩士(2015年)

https://doi.org/10.6346/NPUST.2015.00298

摘要

本實驗室於前期研究中，證實中國南瓜的果實含有降血糖的成分，且降血糖活性成分屬於極性較低的分子，可利用分配萃取法將之濃縮於乙酸乙酯(ethyl acetate；EA)層中。本研究的目的是以第二型糖尿病的動物模型分析南瓜果實EA萃取物於活體中的降血糖效果，方法是以餵食高脂肪食物誘發胰島素抗性，輔以注射streptozotocin(stz)破壞細胞建立第二型糖尿病小鼠模型，再餵食南瓜果實EA萃取物觀察血糖的變化。結果，EA萃取物於老鼠口服葡萄糖耐受性測試中，有減緩血糖上升的效果。餵食EA萃取物23天後，糖尿病鼠的血糖明顯下降，而正常老鼠餵食EA萃取物則無明顯副作用。本實驗的結果證實，南瓜果實的EA萃取物於活體有降血糖效果。另外，以高脂肪飲食輔以STZ注射所建立的第二型糖尿病模型還不夠穩定，需要再最佳化其中的操作條件。

關鍵字

中國南瓜 ； 胰島素抗性 ； 口服葡萄糖耐受性試驗 ； 糖尿病

並列摘要

The fruit of Cucurbita moschata has been confirmed to contain hypoglycemic components, and which can be further concentrated by partitioning between ethyl acetate (EA) and water to distribute them to the EA layer. The purpose of this study is to investigate the hypoglycaemic effect of the EA extract in diabetic animal models. To establish type 2 diabetic models, mice were fed with high-fat diet to induce insulin resistance, followed by injection of streptozotocin (stz) to destroy the pancreatic cell. The mice were then fed with the EA extract of pumpkin for 23 days. Consequently, the EA extract obviously reduced the level of blood in glucose oral glucose tolerance tests, and the fasting blood glucose of EA extract-fed mice was significantly decreased. Meanwhile, normal mice fed with the EA extract did not exhibit any obviously side effect hypoglycaemic. These result support that the EA extract of pumpkin has activity in vivo. However, it was also revealed that the method of using high-fat diet plus STZ injection to diet established by 2 diabetes model not develop type diabetes is not stable, and needs to be further optimized.

並列關鍵字

Cucurbita moschata ； insulin resistant ； oral glucose tolerance test ； insulin tolerance test ； type 2 diabetes

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