神秘果的抗氧化及對STZ誘導的高血糖老鼠血糖調節作用之研究

Studies on the Antioxidant Activity and Plasma Glucose Regulation in STZ-Induced Hyperglycemic Rats of Miracle Fruit

邱毅超(Yi-Chao Ciou)

指導教授 ： 賴宏亮 ； 陳建志

國立屏東科技大學/農學院/生物科技研究所/碩士(2010年)

https://doi.org/10.6346/NPUST.2010.00148

摘要

神秘果 (Synsepalum dulcificum Denill) 屬山欖科 (Saptaceae) 神秘果屬 (Synsepalum) 常綠灌木，原產於熱帶低緯度地區的西非、剛果與迦納一帶，其果肉可以改變味蕾，使酸性味道轉為甜味，可以滿足糖尿病患者或其他代謝症候群所需甜味之變味劑。本篇研究主要目的是使用Streptozotocin (STZ) 誘發高血糖的動物模式，觀察神秘果之降血糖能力。神秘果不同的部位 (外果皮、果實和種子) 與沒食子酸之檢量線推算出總酚的濃度，外果皮 (41.7±11.0 mg gallic acid eq./g D.W.) 為較高，全果 (18.0±3.2 mg gallic acid eq./g D.W.) 和種子 (13.0±2.1 mg gallic allic acid eq./g D.W.) 無明顯差異 (p<0.05) 。神秘果不同的部位和不同萃取方式對於自由基的清除率有不同效果，在 DPPH 自由基清除率上，外果皮在自由基清除率上有顯著的效果，尤其以甲醇萃取物具有最佳的抗氧化能力 (SC50 326.56 μg/mL)。高血糖組中隨機分四組，分別餵食不同的樣品，分為正常組 (NC)、STZ-induced (65 mg/kg) 的糖尿病組 (DM)、糖尿病鼠投予 Glibenclamide (DM+GC 5mg/kg) 組、糖尿病鼠投予神秘果 (DM+MF 0.1 mg/kg) 等四組。誘導四週後測空腹血糖值及葡萄糖耐受性試驗 (oral glucose tolerance test，OGTT)。空腹血糖值餵食神秘果 (0.1 g/kg) 下降了平均降低74 mg/dL。而在口服葡萄糖耐受性試驗在餵食神秘果 (0.1 g/kg) 後，180分鐘間平均下降低了126.7 mg/dL。結果中可以看出，比較組織中細胞葡萄糖轉運蛋白4 (GLUT4) 濃度，神秘果可能對於褐色脂肪、紅肌 (比目魚肌) 和白肌 (伸趾長肌) 細胞 GLUT4 濃度的提升上有作用，其中對於褐色脂肪細胞中 GLUT4 濃度上升有較佳的效果。實驗結果顯示神秘果有降低高血糖的潛力。

關鍵字

糖尿病 ； 神秘果 (Synsepalum dulcificum Denill) ； 總酚 ； DPPH 清除率 ； 葡萄糖耐受性 ； 葡萄糖轉運蛋白 4

並列摘要

Miracle fruit (Synsepalum dulcificum Denill) detected changes of flavors to join the taste buds, transforming acidic taste to sweet. It was used sugar substitute in diabetes. The objectives of this study were to investigate the effects of miracle fruit on the levels of plasma glucose in hyperglycemic rats induced by streptozotocin (STZ). Different parts of the miracle fruit (exocarp, fruit and seeds) and gallic acid calibration curve were used to calculate the concentration of total phenolics, exocarp (41.7±11.0 mg gallic acid eq./g D.W.) was higher, the fruit (18.0±3.2 mg gallic acid eq./g D.W.) and seeds (13.0±2.1 mg gallic allic acid eq./g D.W.) (p<0.05).We detected the DPPH scavenging ability of different parts and different extraction methods of the miracle fruit for free radical effects. The husk in the scavenging rates have significant effects, especially in methanol extracts of the best anti-oxidation (SC50 326.56 μg/mL). The hyperglycemic rats were randomly divided into four groups, four in each, and fed with different experimental diets, part to normal control group (NC), STZ-induced the Diabetes Mellitus group (DM) , hyperglycemic rats diets Glibenclamide (DM+GC 0.5 mg/kg), and hyperglycemic rats diets miracle fruit (DM+MF 0.1 g/kg) respectively. Animals were fed for four weeks. In the response of oral glucose tolerance test (OGTT). Fasting plasma glucose fed miracle fruit (0.1 g/kg) decreased 74 mg/dL. In the oral glucose tolerance test in miracle fruit fed (0.1 g/kg) after 180 minutes on average to lower 126.7 mg/dL. Results show different tissue of glucose transporters 4 (GLUT4) concentration, the miracle fruit can be the inerscapular adipose tissue, soles and extensor digitorum longus muscle cells to enhance concentration of GLUT4, in which the results inerscapular adipose tissue cells increase of GLUT4 concentration. The results showed lower high blood sugar potential of miracle fruit.

並列關鍵字

Diabetes Mellitus ； Miracle fruit (Synsepalum dulcificum Daniell) ； total phenols ； DPPH scavenging ability ； oral glucose tolerance test (OGTT) ； Glucose transporters 4