雙溫區微域加熱聚合酶連鎖反應晶片之設計

Design of Two-Heater Micro-Domain Heating Chip for PCR

王淵駿(Wang Yuanjyun)

指導教授 ： 陳志堅

國立屏東科技大學/工學院/生物機電工程系所/碩士(2010年)

https://doi.org/10.6346/NPUST.2010.00258

摘要

本研究目的是研發在晶片上的微域加熱器。利用軟體CFD-RC模擬設計微域加熱晶片，晶片上設計兩個不同溫度的恆溫區，晶片底部佈置兩組微加熱器，探討兩加熱溫區距離不同，和配合聚合酶連鎖反應（PCR）使用時溫區溫度設定條件不同而中間溫區設置材料不同。在實驗部分也配合聚合酶連鎖反應的反應溫區需要三種不同的反應溫區，利用微機電製程，在晶片上設計兩個不同溫度的恆溫區，晶片底部佈置兩組微加熱器，構成三溫區的恆溫微域加熱區域。以微機電製程方法製作晶片，微域加熱晶片可分成三個部份，包含PDMS流道和玻璃之微域加熱晶片本體、LabVIEW溫度控制程式。兩個微機電製程的加熱裝置形成不同溫度的恆溫區時，利用水冷流道的效果，使晶片表面呈現三個不同溫度的微域恆溫區。為了抑制PDMS流道中氣泡的產生，流道表面以體積比為20%的Tween 20處理，可以大大降低氣泡在流道中形成。

關鍵字

微域加熱 ； 恆溫區 ； 聚合酶連鎖反應

並列摘要

The goal of this research is to develop micro-domain heaters on chips. Using software CFD-RC to simulate the thermal fields of micro-domain heating chip, there are two different constant temperature regions designed on the chip, and the two micro heaters are fabricated at the bottom of the chip. The purpose of this research is to develop micro-domain heaters on chips for applying in polymerase chain reaction. Using MEMS process we fabricate two different heaters on the chip. Three different constant temperature regions are created by means of heat conduction mechanism. In the experiment, the micro-domain heating system mainly consists of three parts: PDMS channel, micro-domain heating chip and LabVIEW control module. The LabVIEW control module is used to manage the temperature of micro-domain heating. By utilizing the waterwall cooling effect, three different temperatures of micro-domain isothermal regions are observed. The formation of air bubbles within the device is suppressed by treating the flow channel with 20% (v/v) Tween 20.

並列關鍵字

Micro-domain heating chip ； Isothermal region ； Polymerase chain reaction

參考文獻

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