臺灣獼猴之猿猴嗜T淋巴細胞病毒之診斷技術建立及感染率初探

Establishment of diagnostic method and investigation of infectious rate of simian T-lymphotropic virus in Formosan macaques (Macaca cyclopis) in Taiwan

林文琦(Wen-Chi Lin)

指導教授 ： 林昭男 ； 邱明堂

國立屏東科技大學/農學院/獸醫學系所/碩士(2014年)

https://doi.org/10.6346/NPUST.2014.00314

摘要

臺灣獼猴 (Macaca cyclopis) 是臺灣唯一的原生特有種非人靈長類動物，其棲地與人類活動範圍高度重疊。由於已有少數血清學研究顯示臺灣獼猴可能有猿猴嗜T淋巴細胞病毒 (simian T-lymphotropic virus, STLV) 感染，且臺灣居民感染人類嗜T淋巴細胞病毒 (human T- lymphotropic virus) 已被證實，因此為探究臺灣獼猴感染人畜共通傳染性之 STLV 的情形，本研究參考Van Dooren等人所發表之文獻，建立半巢式遞減式聚合酶鏈鎖反應 (semi-nested, touchdown polymerase chain reaction) 之STLV前病毒 (provirus) tax 基因序列篩檢技術，並以此技術與市售 ELISA 套組檢測 80 隻臺灣獼猴組織臟器樣本、120 隻臺灣獼猴之EDTA抗凝血液樣本，以及上述 200 隻個體中，128 隻個體的血清樣本。結果顯示 9 隻圈飼與 2 隻野外臺灣獼猴個體中，共 12 件核酸樣本於 PCR 檢測呈 STLV 陽性反應、10 件血清樣本於 ELISA 檢測中呈 HTLV 抗體陽性，個體陽性率為 5.5 % (11/200)。3 個樣本檢出之核酸為首次於獼猴屬個體發現自然感染之第三型 STLV，其餘 9 個核酸樣本則屬於廣泛分布於亞洲與非洲屬獼猴之第一型 STLV。在 7 件陽性組織檢體中，僅生殖系統檢出 2 次，肺臟、骨髓、腸繫膜淋巴結、鼠蹊淋巴結與唾液腺各被檢出 1 次。本研究建立之 STLV 篩檢技術與 ELISA 檢測結果，證實臺灣獼猴之 STLV 感染，並首次於獼猴屬靈長類動物發現自然感染之第三型 STLV。

關鍵字

臺灣獼猴 ； 猿猴嗜T淋巴細胞病毒 ； 聚合酶鏈鎖反應

並列摘要

Formosan macaque (Macaca cyclopis) is the only native species of non-human primate in Taiwan that natural habitat highly overlapped with human activity area. Since previous research revealed the possibility of simian T-lymphotropic virus (STLV) infection in Formosan macaque, and confirmed human infection of human T-lymphotropic virus (HTLV) in Taiwan residents. In order to investigate the infectious status of zoonotic STLV in Formosan macaques in Taiwan, primers of previous studies for tax gene proviral detection had been modified for semi-nested touchdown polymerase chain reaction method establishment. Organ tissues from 80 individuals, EDTA anti-coagulated blood samples from 120 Formosan macaques, and serum samples from 128 individuals among previous 200 individuals had been tested. In 2 wild and 9 captive individuals, 12 samples had STLV tax sequence positive result, and 10 serum samples had HTLV antibody positive result. The overall infectious rate is 5.5% (11/200). The phylogenetic analysis showed 3 neucleic acid samples are STLV-3 which is the first discovery in naturally infected Macaca sp. while the other 9 are STLV-1 that had been commonly found in Asian and Afircan macaques. In 7 positive tissues samples, only reproductive system sample had 2 positive results; lung, bone marrow, mesenteric lymph node, inguinal lymph node and salivary gland had only 1 positive result respectively. In this study, the STLV molecular diagnostic method had been established; the combined molecular and serological results confirmed STLV naturally infections in Formosan macaques.

並列關鍵字

Formosan macaque ； simian T-lymphotropic virus ； PCR

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