食品中新型溴化與氯化阻燃劑分析方法開發與市場肉品監測應用

Development of a New Method for the Monitoring of Novel Brominated and Chlorinated Flame Retardants in Market Meats

許富琳(Hsu Fu-Lin)

指導教授 ： 趙浩然

國立屏東科技大學/工學院/環境工程與科學系所/碩士(2019年)

https://doi.org/10.6346/NPUST201900260

摘要

自從多溴聯苯醚（Polybrominated diphenyl ethers, PBDEs）陸續被各個國家禁用後，NBFRs（Novel brominated flame retardants）及DP（Dechlorane Plus）等新型阻燃劑便作為其替代品引入市場。NBFRs和DP與PBDEs的物化性質相似，顯示對環境和人類有相似的潛在風險。而食物攝入為其進入人體的主要暴露途徑，台灣在食品方面之相關研究及分析方法相當缺乏，故本研究目的為開發食品中NBFRs和DP分析方法，並比較索氏萃取法及QuEChERS兩種萃取法之效果，再利用GC/MS分析肉品樣本濃度，以探討該方法之適用性。本研究以索氏萃取法萃取分析之樣本，其層析圖譜多有一致性，與NIST(National Institute of Standards and Technology)資料庫比對後，其多屬不同種類之脂肪酸，並無觀察到目標化合物；而以QuEChERS法萃取之樣本，在分析後得到之層析圖譜皆無明顯波峰，但在添加內標之樣本經分析後，可觀察到層析圖譜中明顯波峰，皆與NIST資料庫相符，證實QuEChERS萃取法其萃取後樣本基質，並不會對此分析方法造成干擾。本次研究中尚無法確定肉品樣本中NBFRs與DPs含量，但已成功開發以GC/MS分析BEHTBP、BTBPE、DBDPE、EHTBB、syn-DP與anti-DP 6種目標化合物之分析參數。

關鍵字

新型溴化阻燃劑 ； 肉品 ； 索氏萃取法 ； QuEChERS ； 氣相層析質譜儀

並列摘要

Polybrominated diphenyl ethers (PBDEs) have been banned in various countries due to their toxicity and adverse health effects, thus, the use of alternatives such as new flame retardants which include NBFRs (Novel brominated flame retardants) and DP (Dechlorane Plus) have been introduced into the market and has been increasingly used ever since. Due to their similarities with PBDEs, NBFRs and DPs may also pose the same environmental pollution and human health risks. Food intake is one of the main exposure pathways in humans to the alternative fire retardants. However, there is a lack of study regarding the NBFR and DP levels in food in Taiwan which also mirrors the lack of standard analytical methods that can be used for their detection in various food samples. Our study aims to determine an efficient method for the detection of NBFR and DP in meat samples by performing two available extraction methods: Soxhlet extraction and QuEChERS extraction, and afterwards subjecting the extracts for analysis using the gas chromatography/mass spectrometry (GC/MS) and comparing the results to determine which one is better. No NBFR and DP levels were detected in the meat samples upon comparison of the chromatograms with the NIST (National Institute of Standards and Technology) database, except for the different fatty acids while for samples containing internal standards, peak levels of NBFR and DP were observed. The results confirmed that the sample matrix of the QuEChERS extraction method after extraction did not interfere with the analysis. Overall, although NBFR and DP were not detected in the meat samples bought from the Taiwanese market, the analytical standards for six target compounds which include BEHTBP, BTBPE, DBDPE, EHTBB, syn-DP and anti-DP were detected, respectively. Therefore, the QuEChERS method may be a potential extraction method for the determination of NBFRs and DP in food samples. Further studies are suggested to determine the efficiency of Quechers.

並列關鍵字

NBFRs ； Meats ； Soxhlet ； QuEChERS ； GC-MS

參考文獻

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