旋轉平台多標的細胞觀測系統之開發

Development of multi-sample cell observation system with a rotate platform

呂松育(Lu Song-Yu)

指導教授 ： 王耀男

國立屏東科技大學/工學院/車輛工程系所/碩士(2020年)

https://doi.org/10.6346/NPUST202000411

摘要

本研究透過互補性氧化金屬半導體(Complementary Metal-Oxide Semiconductor, CMOS)工業相機整合液態透鏡、光學濾鏡組以及顯微物鏡建置一可自動對焦之影像擷取系統並搭配電動旋轉缸結合3D 列印技術建置一多樣品載臺；建立一套可置於細胞培養箱內旋轉平臺多標的細胞觀測系統，透過LabVIEW圖控程式整合各元件並提供自動化縮時之細胞影像，以降低長時間細胞培養過程之人力成本以及細胞汙染之風險。透過光學系統以及光源之配置，於單一影像擷取系統中實現拍攝明視野以及螢光視野之細胞影像並透過影像處理技術將細胞與背景分離，對細胞成長結果進行量化分析。透過觀測小鼠胚胎細胞以並於培養基中分別添加0.2、0.4、0.6、0.8、1 μg⁄ml 之LPS以及控制組進行對照；從結果中發現到，細胞成長率與添加濃度成反比並分別得到各組分裂指數：60.3 % ~ 65.3 %、53.9 % ~ 58.78 %、52.1 % ~ 55.06 %、46.7 % ~ 51.27 %、42.51 % ~ 45.54 % 以及38.71 % ~ 39.8 %；倍增時間為23.58 ~ 27.01小時、28.15 ~ 32.22小時、31.19 ~ 34.71小時、36.51 ~ 39.61小時、42.18 ~ 45.54小時以及49.17 ~ 50.97小時。

關鍵字

微機電整合 ； 光學系統

並列摘要

In this study, Development of Auto-focusing image capture systeman include complementary metal oxide semiconductor (CMOS) industrial camera of a liquid lens, an optical filter set and an objective lens was proposed, which was constructed an electric rotate motor combined with the multi-sample cell culture platform by the 3D printing technology; development of multi-target cell observation system that can be placed on a rotating platform in the incubator, integration control of various components by the LabVIEW and providing automated time-lapse cell images to reduce long-term cells culture the labor cost and risk of cell contamination. Through the configuration of the optical system and the light source, the cell image of the bright field and the fluorescent field were captured in a single image capture system, and the cell is separated from the background by the image processing technology, the cell growth result was further proposed for the quantitatively analyzed. By observing the mouse embryonic cells with addition of 0.2, 0.4, 0.6, 0.8, 1 μg⁄ml of LPS and the control group to the medium, the results that the cell growth rate was inversely proportional to the concentration of addition and were obtained separately. The division index of each group were 60.3 % ~ 65.3 %, 53.9 % ~ 58.78 %, 52.1 % ~ 55.06 %, 46.7 % ~ 51.27 %, 42.51 % ~ 45.54 % and 38.71 % ~ 39.8 %, and the doubling time were 23.58 ~ 27.01 hr, 28.15 ~ 32.22 hr, 31.19 ~ 34.71 hr, 36.51 ~ 39.61 hr, 42.18 ~ 45.54 hr and 49.17 ~ 50.97 hr. Over all, the results presented in this study confirm, that the LPS for mouse cells can produce a immune response and slow down the growth rate of cells. The system can provide long term observation for cell culture in vitro culture, providing bright the fluorescent of cell image.

並列關鍵字

CMOS ； MEMS integration ； optics system

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