利用生物偵測法調查室內灰塵中類戴奧辛與發炎效應及其對健康之影響

Using bioassay to investigate dioxin-like and inflammatory responses in indoor dust and associated health effects

林恩賜(Lin En-Tzu)

指導教授 ： 黃國林 ； 趙浩然

國立屏東科技大學/工學院/環境工程與科學系所/碩士(2021年)

https://doi.org/10.6346/NPUST202100369

摘要

現代大多數人大約有 80-90% 的時間會待在室內環境中，但室內環境中由於建築材料、電子產品以及清潔產品中排放許多化合物，例如類戴奧辛化合物、發炎物質等等，這些化合物均有研究表明會對人體產生明確的危害。大部分的化合物的分析需要透過化學法HRGC/HRMS(High resolutjon gas chromatography-High resolution mass spectrometry)，但需耗費大量時間及成本。因此本研究想藉由生物偵測法，調查南部地區安養院、中醫診間以及健身房的室內灰塵中類戴奧辛毒性當量濃度以及發炎反應，並進一步評估其對健康風險之影響。研究結果發現在Ad-DR bioassay檢測室內灰塵中類戴奧辛毒性當量濃度中以中醫診間最高(4.39±2.67 pg BEQ/g)，其次是健身房(2.54±2.40 pg BEQ/g)，最低則是安養院(1.92±4.27 pg BEQ/g)。利用NF-κB bioassay進行發炎反應之濃度研究中，結果發現最高為健身房(1.34±1.01 pg TNF-α/g)，其次是安養院(1.33±0.969 pg TNF-α/g)，最低是中醫診間(1.06±1.04 pg TNF-α/g)。研究發現影響到類戴奧辛毒性當量濃度的因素有室內電器產品的數量、室外移動污染源數量及與固定污染源的距離，且推測露天燃燒也可能會對其有一定貢獻程度。另外發炎潛能的部分則在城市地區中呈現較高濃度，但與移動及固定污染源間距離並無相關性。在計算室內灰塵對人體之健康風險評估中，發現每日攝入量、非致癌風險以及致癌風險均未超出建議值，顯示在所有場域中人體經由室內灰塵攝入，均不會對人體產生非致癌風險以及致癌風險。上述實驗證實生物偵測法可應用於類戴奧辛濃度、發炎效應及健康風險評估。

關鍵字

室內灰塵 ； Ad-DR bioassay ； NF-κB bioassay ； 風險評估

並列摘要

Most people,on average, spend approximately 80-90% of their time indoor, where concentrations of different environmental chemicals, such as dioxin-like compounds, inflammatory substances.The building materials, electronic products, furnishings, and cleaning products have been identified as possible sources for potentially hazardous substances in indoor air. High resolutjon gas chromatography-High resolution mass spectrometry (HRGC/HRMS) is the most suitable technique for the analysis of most compounds, but it takes a lot of time and cost. Therefore, the current study was aimed to investigate dioxin-like pollution status and inflammatory responses in indoor dust using bioassay as well as estimate health risk. The indoor dust from southern nursing homes, Chinese medicine clinics, and gym has been collected.The Ad-DR bioassay showed the indoor dust had the highest dioxin-like BEQ concentration in Chinese medicine clinics (4.39±2.67 pg BEQ/g), followed by gym (2.54±2.40 pg BEQ/g), and the lowest in nursing homes (1.92±4.27 pg BEQ/g). The NF-κB bioassay found that the highest concentration of inflammatory response was the gym (1.34±1.01 pg TNF-α/g), followed by nursing home (1.33±0.969 pg TNF-α/g), and the lowest was the Chinese medicine clinic (1.06±1.04 pg TNF-α/g). The number of indoor electrical products, outdoor mobile pollution sources, and the distance to stationary pollution source has influenced the dioxin-like BEQ concentration.However, there was no significant correlation between the concentration of inflammatory and the distance to stationary pollution source. Potential health risk assessment also showed the daily intake, non-carcinogenic risk, and carcinogenic risk of indoor dust in nursing homes, Chinese medicine clinics, and gyms were in a safe range. The findings demonstrate the bioassay may serve as a technique for the analysis of dioxin-like pollution status and inflammatory responses as well as an estimated health risk.

並列關鍵字

Indoor dust ； Ad-DR bioassay ； NF-κB bioassay ； Risk assessment

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