臺灣鸚鵡玻那病毒之研究

Study of Avian Bornavirus of Parrot in Taiwan

李萬鵬(Lee Wan-Peng)

指導教授 ： 吳弘毅

國立屏東科技大學/獸醫學院/獸醫學系所/碩士(2018年)

https://doi.org/10.6346/THE.NPUST.VM.001.2018.D07

摘要

臺灣鸚鵡疾病調查，鮮少探討禽玻那病毒(Avian bornaviruses)感染造成的腺胃擴張症(Proventricular dilatation, PDD)，其特徵性組織病變為其為淋巴細胞─漿細胞性消化道周邊神經節炎及腦脊髓炎，因此又可稱禽玻那神經節炎(Avian Bornaviral Ganglioneuritis, ABG)。本次主要針對臺灣所飼養之240隻鸚鵡之嘔吐物樣本進行禽玻那病毒的調查和分析其中6隻完整病例之序列。實驗室檢查包括反轉錄聚合酶鏈式反應(RT-PCR)檢測病原和6隻完整病例的基因定序比較其M基因突變點和組織切片觀察其病變。送檢完整大體病例的大金黃錐尾鸚鵡、琉璃金剛鸚鵡及非洲灰鸚鵡…等有六隻呈陽性反應，且組織病理上具有非化膿性─淋巴細胞─漿細胞性上消化道神經節炎之病灶。病例PC14-460序列比對結果顯示於第97個核甘酸發生點突變；病例PC14-466與PC15-483序列片段於第52、64、151、187、199、252個核甘酸發生點突變；病例PC14-466與PC15-483於第252個核甘酸突變點，造成轉譯的胺基酸改變，由原本的離胺酸轉變為精胺酸。在嘔吐物的收集共有240個樣本，玻那病毒發生率為19.6%，各年齡所占比例，1日至6個月以下鸚鵡比例占4.2%，6個月至3年以下鸚鵡比例占5.8%，3年至7年以下鸚鵡比例占4.6%，7年至30年以下鸚鵡比例占5.0%。在各季節的分布情形，2月至4月(春季)占4.2%，5月至7月(夏季)占5.8%，8月至10月(秋季)占13.3%，11月至1月(冬季)占11.7%。玻那病毒在繁殖場的發生率較高。玻那病毒在各鸚鵡種所占比例，灰鸚鵡占21%、琉璃金剛占17.9%、大金黃錐尾鸚鵡26.2%、巴丹15.6%及其他鸚鵡種16.7%。

關鍵字

鸚鵡 ； 腺胃擴張症 ； 禽玻那病毒 ； 聚合酶鏈鎖反應 ； 定序

並列摘要

In Taiwan, Disease Investigation of parrot rarely mentioned to proventricular dilatation disease (PDD) caused by avian bornaviruses. Its characteristic tissue lesions are lymphocytic-cytoplasmic peri-intestinal peripheral ganglionitis and encephalomyelitis, which can also be referred to as avian bornaviral ganglioneuritis (ABG). In this paper, we focused on the investigation of avian bornaviruses for vomit samples from 240 parrots in Taiwan, and analyze the sequence of 6 complete cases. Our laboratory examination including RT-PCR for the detection of pathogens and gene sequencing in 6 intact cases, to compare M gene mutations and observe the tissue section lesions. Six of the complete cases, including Guaruba guarouba, Ara ararauna, and Psittacus erithacus etc. that are positive reaction and these cases all have PDD. The sequence alignment of PC14-460 showed a point mutation in the 97th nucleotide; the PC14-466 and PC15-483 sequence fragments had point mutations in 52, 64, 151, 187, 199, 252 nucleotides; the cases PC14-466 and PC15-483 were the 252nd nucleotide mutation point, resulting in the translation of the amino acid changes, from the original lysine to arginine. A total of 240 samples were collected for vomiting, the frequency of the Bonavirus was 19.6%. Percentage of all ages, parrots accounted for 4.2% from 1 to 6 months, parrots from 6 months to 3 years is 5.8%, from 3 to 7 years is 4.6%, and from 7 to 30 years is 5.0%.The seasonal distribution ranges from 4.2% in February to April (spring), 5.8% in May to July (summer), 13.3% in August to October (fall), November to January (winter) Accounted for 11.7%. The frequency of Bonavirus in the breeding ground is high. The proportion of Bonavirus in each parrot species was 21% in Psittacus erithacus, 17.9% in Ara ararauna, 26.2% in Guaruba guarouba, 15.6% in Cockatoo, and 16.7% in other parrot species.

並列關鍵字

parrot ； proventricular dilatation disease ； Avian bornaviruses ； PCR ； sequence

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