越南第二型犬小病毒之基因分型和遺傳特徵

Genotyping and Genetic Characterization of Canine Parvovirus Type 2 in Vietnam

黄明(Hoang Minh)

指導教授 ： 林昭男 ； 邱明堂

國立屏東科技大學/獸醫學院/獸醫學系所/博士(2020年)

https://doi.org/10.6346/NPUST202000537

摘要

自從1978年發現以來，第二型犬小病毒（CPV-2）已被認為是犬隻非常重要的病原之一，因為它會造成幼犬高致死率的嚴重腸炎。CPV-2屬於小病毒科，近年來發現有三種基因型別，分別為CPV-2a、CPV-2b及CPV-2c。本研究中，我們通過SimpleProbe® 即時PCR以及DNA定序分析來取得準確及敏感性高的CPV-2基因分型結果。利用SimpleProbe® 即時PCR以及DNA定序分析來進行CPV-2基因分型。接著利用SimpleProbe® 即時PCR來鑑別2016年11月至2018年2月來自越南北、中、南部疑似CPV-2感染之260份直腸拭子檢體。本研究提供證據顯示CPV-2c為越南主要盛行之基因型別。親源分析結果顯示，越南的CPV-2c與亞洲的CPV-2c有共同的演化祖先。此外，序列分析發現有6株小病毒與貓小病毒（FPV）相似，親源分析也顯示該VP2基因全長與FPV相似。而這些與FPV相似的病毒株在VP2蛋白上有Thr101突變。本研究發現Ile101突變為Thr101改變了表面核心受體結合位置的大小、蛋白質接觸電位以及蛋白質的帶電荷。這也是首次提供可靠證據證明FPV可能對越南犬隻造成威脅的研究。
關鍵字：Canine parvovirus type 2, Genotype, SimpleProbe® real-time PCR, Vietnam, SimpleProbe®, FPV

關鍵字

第2型犬小病毒 ； 基因型 ； 越南 ； SimpleProbe® 即時定量 PCR ； 貓泛白血球減少症 ； SimpleProbe®

並列摘要

Since its identification in 1978, Canine parvovirus type 2 (CPV-2) has been considered a pathogen of great importance in the canine population because it causes severe enteritis with high mortality rates in pups. CPV-2 is a virus belonging to the family Parvoviridae. Currently, there are three described antigenic variants (CPV-2a, CPV-2b, and CPV-2c). In this study, we compared CPV-2 genotyping results obtained by SimpleProbe® real-time PCR and DNA sequencing analysis to identify the accuracy and sensitivity of these methods. CPV-2 genotyping was performed by SimpleProbe® real-time PCR and DNA sequencing to compare results. The SimpleProbe® real-time PCR assay was then used to identify strains of 260 rectal swab samples with suspected CPV-2 infection from Northern, Central and South Vietnam from November 2016 to February 2018. The present study provides evidence that CPV-2c is the most prevalent variant in Vietnam. Phylogenetic analysis demonstrated that the recent Vietnamese CPV-2c isolates share a commen evolutionary origin with Asian CPV-2c strains. In addition, Sequence analysis of the 6 parvoviral strains identified the species as being similar to FPV. Phylogenetic analysis demonstrated that the complete VP2 genes of the strains are similar to those of Feline panleukopenia virus. The FPV-like strains contain a Thr101 mutation in the VP2 protein, which is different from prototype FPV strains. Our data provide evidence for the existence of changes in the charge, protein contact potential and molecular surface of the core of the receptor-binding size with an Ile101 to Thr101 mutation. This is also the first study to provide reliable evidence that FPV may be a threat to the Vietnamese dog population.
Keywords: Canine parvovirus type 2, Genotype, SimpleProbe® real-time PCR, Vietnam, SimpleProbe®, FPV

並列關鍵字

Canine parvovirus type 2 ； Genotype ； SimpleProbe® real-time PCR ； Vietnam ； SimpleProbe® ； FPV

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