模擬移動床分離果寡糖之研究

本研究主要在利用模擬移動床(Simulated Moving Bed, SMB)移除台糖果寡糖漿中的單醣及蔗糖。藉由移除葡萄糖、果糖、以及蔗糖等高升糖指數的成分，以提高蔗果三醣(1-Kestose)以及蔗果四醣(Nystose)的含量。本研究首先使用單柱層析調查果寡醣漿主要成分在不同樹脂的滯留行為，結果發現UBK555樹脂具有最好的分離效果，其中滯留性從高到低依序為：果糖>葡萄糖>蔗糖>蔗果三醣>蔗果四醣。利用單柱層析結果並輔以三角形理論，本研究設計一系列的模擬移動床實驗條件，結果發現在連續三天的操作下，蔗果三醣與蔗果四醣的純度可以從56.0%提升到85.5%，回收率可達71.1%。如果將進料濃度從100 g/L調降至20 g/L，則發現果寡醣的純度可達到94.3%，回收率為54.0%，顯示模擬移動床可以有效移除原料中的單醣及部分蔗糖。本研究除了證實模擬移動床在純化果寡糖的可行性之外，更成功利用三角形理論建立可分離的操作條件以及說明實驗結果，可提供未來放大設計與建立生產操作條件的參考之用。

關鍵字

無

並列摘要

This study aimed to remove fructose, glucose, and sucrose in a commercial fructooligosaccharide syrup from Taiwan Sugar Corporation to increase the content of 1-kestose and nystose. Single-column chromatography was firstly applied to investigate the retention behavior of major components in the syrup. It was found that UBK555 resin can effectively separate the fructooligosaccharide from the syrup, and the order of retention follows fructose > glucose > sucrose > 1-kestose > nystose. By applying the Triangle theory, a series of experiments were designed and conducted. It was found that the purity of 1-kestose and nystose in the syrup can be increased from 56.03% to 85.5% with 71.1% of recovery of the fructooligosaccharide for an experiment with three-day operation. It was also found that the purity of 1-kestose and nystose reached 94.3% with 54.0% of recovery, if the feed concentration was decreased from 100 g/L to 20 g/L. The experimental results show the feasibility for the separation of fructose, glucose, and sucrose from the syrup by using a SMB and the application of the Triangle theory to find the operation conditions to separate the fructooligosaccharide. Furthermore, this study provides useful information for future scale-up design and its operation conditions.