- 學位論文
探討不同固態培養基成份對蛹蟲草成長之影響
Effect of medium composition for the solid culture of Cordyceps militaris
摘要
藥用菇類因具有藥用價值而被人們所喜愛，但因數量稀少故價值較 貴。蛹蟲草作為中藥材，在中國有悠久的歷史，於《新華本草綱要》記載其「性平、味甘，有補精髓、益肺腎，並能止血化痰等功效」。近年來研究發現蛹蟲草的藥用功效、保健價值及生物活性與冬蟲夏草相近，其藥理活性，包括抗發炎、抗腫瘤、補腎氣、保護肝臟等，目前已核准蛹蟲草中的活性物質蟲草素為急性白血病之用藥。蛹蟲草可用人工培養的方式培養，有助於商業量產與發展。因此本研究以固態培養方式培養蛹蟲草菌絲體，並嘗試加入不同添加物及調整生長環境來探討對蛹蟲草固態培養的影響。實驗結果發現培養基配方4(HKS3)為最佳的蛹蟲草菌絲體之固態培養基，其生長環境調控以室溫及不照光為佳，這樣的培養條件，有利於蛹蟲草菌絲體成長，並有利於未來將蛹蟲草菌絲體萃取出有效的生物活性成份製成藥物或保健食品。
並列摘要
People like Cordyceps militaris because of its medicinal value, but due to scarcity, it is quite expensive. As one kind of traditional Chinese medicine, Cordyceps militaris has a long application history in China, from the record of "Xinhua Compendium of Materia Medica", it is neutral in nature, sweet in taste, it has the function of nourishing the kidney and lung, benefiting pneuma, stop bleeding and reducing phlegm. Recent studies find that Cordyceps militaris is similar to Cordyceps sinensis in medicinal value, health care value,biological activity and pharmacological activity include anti-inflammatory, anti-tumor, tonifying kidney, protecting liver, sex enhancer and so on. Cordycepin, at present an active compound found in Cordyceps militaris is approved to be used for treating acute leukemia. We can use the way of artificial rearing to culture the Cordyceps militaris that is expected to improve the production yield and development of the commerce. Therefore this study aimed to cultivate Cordyceps militaris by the method of solid state culture and to add different additives and adjust the growing environment and to discuss their effect to solid-state culture. The study results show that the formula of culture medium4(HKS3) is the best solid culture medium of Cordyceps militaris mycelium and the best growing environment is under room temperature and without visible light illumination. With these culturing conditions, growth of mycelia of Cordyceps militaris can be enhanced. In future, mycelia could be utilized for the extraction of active cordycepin and processed into medicine or health supplement.