透過微流體系統與內/外部凝膠化合成用於生物應用之氫氧化鋇複合顆粒

Synthesize Barium Hydroxide Composite Particles for Biomedical Applications Through Microfluidic System and Internal/External Gelation

史奕軒(Yi-Syuan Shih)

指導教授 ： 楊智惠

共同指導教授 ： 黃耿祥(Keng-Shiang Huang)

義守大學/醫學院/生物科技學系/碩士(2021年)

摘要

「微流體晶片」在學界與業界都十分受到重視，其製備出之微粒粒徑具有可控性與可調控為最大特點。而氫氧化鋇能在人工牙根中可作為抑菌劑，也有相關研究用於骨水泥中來加強生物相容性，本研究利用實驗室所長，不同於以往製備氫氧化鋇的舊有方法，利用新式且簡便快速的方法，來達到離子交換並製成粒徑均一的氫氧化鋇微粒，且進一步經由針滴液滴與微流體晶片能調控製備出1.2 mm ~ 200 μm之間不同粒徑尺寸。本研究再藉由FTIR、XRD分析微粒的官能基與特徵峰，SEM/EDS、MTT來分析外觀、內部結構及表面鋇含量與生物相容性，最後再經由ICP-MS檢測其純度。分析結果顯示出使用本研究方法製備出的氫氧化鋇微粒不但粒徑均一且純度較高，而且同時也具備極佳的生物相容性，經由本研究期待可以使氫氧化鋇微粒在生物醫學應用上更具廣度。此外若經由內部凝膠化製得之氫氧化鋇微粒，發現到其可含有微泡 (孔洞) ，其尺寸約可調控在180 ~ 200 μm之間，而微氣泡微粒可作為超聲波應用和靶向藥物遞送的媒介甚至是作為細胞支架等用途，深入研究將可期運用在更多醫學工程與醫藥領域上的用途。

關鍵字

氫氧化鋇 ； 微流體 ； 生物相容性 ； 內部凝膠 ； 微氣泡微粒

並列摘要

"Microfluidic chips" are highly valued in the academic and industry circles. The particle size of the microfluidic chips prepared by them is controllable and adjustable. Barium hydroxide can be used as a bacteriostatic agent in artificial tooth roots. There are also related studies used in bone cement to enhance biocompatibility. This study uses the laboratory's expertise, which is different from the traditional method of preparing barium hydroxide in the past. The method uses a new and simple and rapid method to achieve ion exchange and produce barium hydroxide particles with uniform particle size, and further regulate and prepare 1.2 mm ~ 200 μm different particle sizes through needle droplets or microfluidic chips. In this study, the functional groups and characteristic peaks of the particles were analyzed by FTIR and XRD, and the appearance, internal structure, and surface barium content and biocompatibility were analyzed by SEM/EDS and MTT. Finally, the purity was tested by ICP-MS. The analysis results show that the barium hydroxide particles prepared by this research method not only have uniform particle size, but also have excellent biocompatibility. Through this research, it is expected barium hydroxide particles can potential be used in Biomedical applications. In addition, if the barium hydroxide particles prepared by internal gelation, it is found that they may contain microbubbles (pores) were observed inside, the size of which can potential be adjusted between 180 ~ 200 μm, and the microbubble particles can be used for ultrasonic applications and targeting The medium of drug delivery is even used as a cell scaffold. In-depth research will be expected to be used in more medical engineering and pharmaceutical fields.

並列關鍵字

Barium hydroxide ； Microfluidic chip ； Biocompatibility ； Internal gelation ； Microbubbles

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