材料表面的冷電漿聚合及表面接枝聚合水膠在生物醫學的應用

Surface Modification of Materials by Cold Plasma Treatment and Subsequently Grafting Polymerization Hydrogel for Biomedical Applications

洪翠禪(Tsuei-chan Hung)

指導教授 ： 陳克紹

大同大學/工程學院/材料工程學系所/碩士(2007年)

摘要

本研究主要使用冷電漿改質材料表面及UV 光接枝聚合製備感溫性
水膠，以探討此類材料在生物醫學上之應用例如細胞貼附性、互穿式
(Inter-penetrating Net-Work, IPN)感溫性水膠和奈米金粒子表面接枝固定
感溫性水膠聚異丙基丙烯醯胺(poly(N-isopropyl acrylamide),PNIPAAm)。
利用冷電漿聚合方法，於聚苯乙烯(polystyrene, PS)表面上沉積醇類
或有機矽烷類高分子膜，測試其細胞貼附性。以異丙醇電漿處理表面
後，使PS變成親水性，且由巨噬細胞與黑色素細胞做培養，其貼附性不
亞於市販細胞培養皿。另外以UV光接枝聚合聚丙烯醯胺(poly acrylamide,
PAAm)，再利用PAAm水膠的彭潤性質吸入異丙基丙烯醯胺(NIPAAm)單
體，製備互穿式三明治狀水膠IPN-(PAAm/PNIPAAm)，由於PNIPAAm具
特殊的臨界溫度(約32℃)轉換特性，藉此代替人工肌肉組織，確認超音
波治療的聚焦點。
本研究最後結合前兩項技術，以電漿處理產生自由基之方法，將含
羧基硫醇之11-Mercaptoundecanoic acid (MUA)修飾後的金奈米粒子，使
用冷電漿輝光於Nano-Au(mua)表面產生自由基，即可以UV 光接枝將水
膠單體分子結合於金奈米粒子上。穩定、快速且方便的將金奈米粒子表
面進行修飾。結果得知Nano-Au(mua)為酒紅色，以氧電漿處理後再接枝
上感溫性PNIPAAm，呈紫紅色且Nano-Au(mua)-g-PNIPAAm 有溫度感應性。

關鍵字

電漿處理 ； 接枝聚合 ； 水膠 ； 奈米金

並列摘要

The major of this study is use cold plasma modification on substrate
and UV light grafting polymerization thermosensitive hydrogel. To confer
the application of this kind of material on biomedical, such as cell
attachment, Inter-penetrating Net-Work (IPN) thermosensitive hydrogel and
surface grafting poly(N-isopropyl acrylamide) (PNIPAAm) on gold
nanoparticle.
To make use of cold plasma copolymerization to deposition alcohol or
organosilicon polymer film on polystyrene (PS), and test the cell adherence.
PS change into hydrophilicity, and the cell adherence as well as tissue culture
polystyrene (TCPS) when cell culture macrophage and melanocyte after
isopropanol (IPA) plasma deposition. Besides graft poly acrylamide (PAAm)
by UV light, use the swelling character of PAAm to suck NIPAAm monomer
and prepare the sandwich like hydrogel IPN-(PAAm/PNIPAAm). Due to
there are still existence special characteristic of Lower Critical Solution
Temperature (LCST) about 32 of PNIPAAm. ℃ For this reason, take the
place of artificial musculature as a new model for irradiation experiments
using focused ultrasound.
The final of this investigate combine the front of two techniques, use
plasma treatment to produce free radicle. To take gold nanoparticle use cold
plasma glow discharge to produce free radicle after embellish of
11-Mercaptoundecanoic acid (MUA) that contact with carboxyl and thiol.
Subsequently use UV light grafting hydrogel monomer on gold nanoparticle.
Embellish gold nanoparticle surface steady, fast and convenient. As a result,
the color of Nano-Au(mua) is red wine, after O2 plasma treatment and then
grafting thermosensitive PNIPAAm is purplish red and
Nano-Au(mua)-g-PNIPAAm still have thermosensitive property.

並列關鍵字

plasma treatment ； photo-induced grafted polymerization

參考文獻

[50] 林宏聲，蛋白質於金奈米粒子表面吸附之探討，中原大學化學工程

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