香蕉皮萃取物及其有效成份對於急性發炎之緩解效果

The remission of acute inflammation and the separation of active principle for Banana peel extract

陳甲樺(Jia-Hua Chen)

指導教授 ： 洪永瀚

義守大學/理工學院/化學工程學系暨生物技術與化學工程研究所/碩士(2017年)

摘要

許多疾病發展與免疫調整異常有關，過去並未有相關文獻探討香蕉皮的免疫調節功能，故本研究欲探討香蕉皮正丁醇萃取物(Banana peel n-butanol extract, BPE)在急性發炎模式下如何透過免疫調節達到抗炎之效。本研究將BALB/c小鼠分為四組，分別為控制組、低高劑量BPE組(42；166mg/kg BW)以及正控制組-PDTC組；在各組小鼠餵食水或樣品1週後，以腹腔注射LPS(Lipopolysaccharide)誘發急性發炎，在3小時後犧牲小鼠，收集血液、臟器及淋巴結進行各項免疫生化分析。結果顯示，相較於控制組，高劑量BPE組血液中IL-6與TNF-α明顯降低，與正控制組PDTC結果相同；腹腔細胞分泌之IL-6量也顯著降低；而觀察脾臟刺激T細胞，IFN-γ及IL-6分泌量亦明顯降低；而IL-4及IL-10生成量卻反而些微增加，顯示BPE調整Th1/Th2平衡而降低發炎反應；在調節T細胞(Regulatory T cell)表達方面，高低劑量及正控制組都有明顯下降且具顯著差異。
本研究分析BPE中其成份之抗炎效果發現，5-羥甲基糠醛(5-hydroxymethylfurfural)、愈創醇(guaiol)、油酸醯胺(oleamide)濃度100μM可抑制LPS刺激巨噬細胞株分泌IL-6及TNF-α之能力。綜合上述結果說明BPE及其分離成分透過降低促發炎細胞激素的生成，能達到抑制LPS誘發之急性發炎反應。

關鍵字

香蕉皮正丁醇萃取物 ； 發炎 ； 細胞激素 ； T細胞 ； 5-羥甲基糠醛 ； 愈創醇 ； 油酸醯胺

並列摘要

Because of the development of many diseases are related to the immune abnormalities, and there is no relevant literature that explored the immune system of banana peels in the past, so this research is to explore the remission of acute inflammation and the separation of active principle for Musa paradisiacal peel n-butanol extract(BPE).In this study, we divided the BALB/c mice into four groups; the control group, the low-dose and the high-dose BPE group (42; 166mg / kg BW) and the positive control group-PDTC group. After a week of feeding water and sample for each group, we do the intraperitoneal injection of LPS (Lipopolysaccharide) to induced acute inflammation, the mice will sacrifice 3 hours later, and we collected the blood, organs and lymph nodes for immuno-biochemical analysis.The results shows that compare to the control group, the levels of blood IL-6 and TNF-α in the high-dose BPE group were significantly decreasing, which is the same as the control group. The amount of the IL-6 peritoneal cell secretion was also significantly decreasing, and when observing the stimulation of the spleen to T cell, we found that the secretion of IFN-γ and IL-6 were also decreasing. But instead, the formation of IL-4 and IL-10 has increased slightly. The research shows that BPE adjust the balance of Th1/Th2 and reduced the response of inflammatory. And in the adjustments of T cells (Regulatory T cell), the high-dose, low-dose group and the positive control group were significantly decreased and were having significant differences.
In this research, we analyzed the anti-inflammatory effects of the components in BPE and found that, 5-hydroxymethylfurfural, Guaiol and the oleamide concentrations of 100μM can inhibited the ability of LPS stimulating macrophage to secret IL-6 And TNF-α.
This research shows that BPE and its isolated components can inhibit LPS to induce acute inflammation by decreasing the formation of the inflammatory cytokine.

並列關鍵字

Musa paradisiacal peel n-butanol extract(BPE) ； inflammation ； cytokines ； Regulatory T cell ； 5-hydroxymethylfurfural ； guaiol ； oleamide

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