酵母菌 ( Saccharomyces rouxii ) 加壓供氧發酵生產耐糖因子之研究

Production of glucose tolerant factor by yeast ( Saccharomyces rouxii ) fermentation in pressurized air system

江佳閎(Chia-hung Chiang)

指導教授 ： 陳齊聖

朝陽科技大學/理工學院/應用化學系/碩士(2012年)

摘要

本研究探討以耐糖度最高且耐高滲透壓之酵母菌( Saccharomyces
rouxii ) 生產葡萄糖耐糖因子，生產菌株篩選用來自醬醪所篩選出，菌株篩選以比生長速率、鉻離子消耗速率為篩選依據指標，所得菌株做為各種操作變因之探討。本研究比較靜置發酵與噴流供氧( Sparging )方式以及間歇加壓供氧方式，溶氧動力學顯示在2.5-10 psi 壓力情況下，以噴流供氧( Sparging )方式測得水中飽和溶氧量5 秒鐘即可達到飽和 (6 ppm)。間歇加壓供氧方式所需較長時間為20 分鐘即達飽和。間歇加壓供氧方式可避免發酵過程中，噴流供氧所產生之起泡現象與添加酵泡劑之困擾。考慮酵母菌生長速率倍增時間 ( doubling time )約為1~2 小時，間歇加壓供氧方式培養為可行之選項。本研究所得之結論，利用間歇加壓供氧方式模擬發酵培養，
最佳培養條件：發酵液500 mL、接菌體積1 %、培養溫度30 ℃、起始酸鹼值4.0、Cr3+濃度100 ppm、葡萄糖15 % 通氣壓力2.5 psi 、通氣時間 20 分鐘、洩壓 3 秒鐘；32 h 後，Cr3+與葡萄糖濃度均降至零，酵母菌細胞為2.5×108 CFU/mL。

關鍵字

葡萄糖耐糖因子 ； 酵母菌 ； 間歇加壓供氧

並列摘要

This research investigated the production of glucose tolerance factor (GTF)by a highly osmotic and sugar tolerant yeast (Saccharomyces rouxii). The strain
was screened and isolated from soy sauce mash. Strain selection was based on relative specific growth rate and chromium consumption rate. The obtained strain was used for studying various operation variables. The dissolved oxygen
(DO) in static culture, air sparging and intermittent pressurized air of operation modes was compared. Results showed that, at room temperature, DO could reach saturation (6 ppm) within 5 sec, while intermittent pressurized air took 20 min to reach saturation. Using intermittent pressurized air system, bubbling and the need of antifoaming agent, as caused by sparging air, can be
avoided.Considering the doubling time of yeast (1~2 h), the intermittent pressurized air system is a practical option. Experimental results showed that using intermittent pressurized air system, for a working volume of 500 mL,
initial 100 ppm of Cr3+ and 15% glucose were completely consumed after 32 h. The culture temperature was 30 °C, initial pH was 4.0, and the air pressure was 2.5 psi. The fermenter was pressurized for 20 min and then released for 3 sec. The final yeast concentration was 2.5×108 CFU/mL.

並列關鍵字

glucose tolerance factor ； yeast ； intermittent pressurized air system

參考文獻

蘇遠志、黃世佑（1994）微生物化學工程學。華香遠出版社。

呂菁，戴東原（1991）妊娠型糖尿病。當代醫學 18:60-62

唐正乾（2001）糖尿病藥物的新選擇。藥學雜誌 17:141-147

蕭淑華，吳達仁（2000）第2 型糖尿病藥物治療之新進展。臺灣醫學 4:80-85

內分泌暨糖尿病學會會訊11:1-4