魯式酵母菌發酵生產菌體與胞內GTF之供氧策略研究

Oxygenation Stratgies in Zygosaccharomyces rouxii fermentation for biomass and intracellular GTF productions

楊銘輝(MING-HUI YANG)

指導教授 ： 錢偉鈞

朝陽科技大學/理工學院/應用化學系生化科技碩博士班/博士(2016年)

摘要

分子氧之供給策略，對於微生物發酵，非常重要。魯氏酵母菌，為工業應用之重要菌株。本研究探討溶氧動力學對發酵液溶氧之影響，間歇加壓供氧對細胞生長之影響，以及供氧方式對魯氏酵母攝取發酵液中三價鉻離子之影響。實驗結果顯示，壓力對溶氧之影響模式，符合亨利定律。發酵液中之葡萄糖影響飽和溶氧，與溶氧速率。間歇加壓供氧，因跨膜壓差之助，增加胞內溶氧，與氧利用效率，發酵之菌體產率高於連續供氧。供氧充足，魯氏酵母菌不產生乙醇，亦不攝取發酵液中之鉻離子，生產胞內GTF之供氧策略，與生產菌體不同。

關鍵字

魯氏酵母菌 ； 批次發酵 ； 供氧 ； 溶氧動力學 ； 耐糖因子

並列摘要

Strategy of supplying molecular oxygen is very important in microbial fermentation. Zygosaccharomyces rouxii is a yeast strain of many applications in the biochemical industries. This research investigated the dissolving dynamics of molecular oxygen in aqueous fermentation medium, intermittent pressurized oxygenation for cell production, and the effect of oxygen supplement on the uptake of trivalent chromium in the medium by the cell. Experimental results revealed that Henry’s Law is applicable in the ranges of pressure and glucose concentration adopted in this study. Intermittent pressurized oxygenation facilitated the cross-membrane oxygen transfer and enhanced growth efficiency. Cr3+ uptake to synthesize intracellular GTF needs anaerobic environment. Experimental results revealed high efficiency of GTF in terms of glucose metabolism and growth rate.

並列關鍵字

Zygosaccharomyces rouxii ； stirred tank bioreactor ； oxygen dissolving ； dynamics ； GTF

參考文獻

江佳閎 (2012)「酵母菌(Saccharomyces rouxii)加壓供氧發酵生產耐糖因子之研究」，碩士論文，朝陽科技大學應用化學系，台中。

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