牛樟芝為台灣是特有真菌,別稱為樟芝或牛樟菇。牛樟芝的主要的生理活性成分為三萜類,據相關報導指出三萜類有各種生理活性,包括了具有抗癌和抗發炎的潛力。三萜類化合物是牛樟芝萃取物中苦味成份的來源。其中牛樟芝三萜類依相關報導中主要包括了麥角甾烷(Ergostane)和羊毛甾烷(Lanostane)兩大類,然而牛樟芝中活性化合物的含量與種類取決於收穫的時間、萃取方式及培養條件;因此,如何檢測牛樟芝活性成分與含量是很重要的事。為了評估牛樟芝的三萜類的分子量,在這項的研究中,我們針對牛樟芝內Dehydroeburicoic acid、15α-acetyldehydrosulphurenic acid、Antcin B、Zhankuic acid C、Antcin A、Antcin C and Antcin K這七個具有生理活性的三萜類化合物開發了一個準確、快速的LC-ESI-MS/MS的分析方法。 其LC-ESI-MS/MS優化條件如下:在負離子的模式下掃描,偵測範圍為150-700m/z,自動注射流速為5μL/min,毛細管溫度為400℃,氮氣流速為15arb,電噴灑電壓為3kV,然而麥角甾醇與羊毛甾醇二次撞擊之電壓大小分別為35% - 30%、39% - 42%。
Antrodia camphorata (AC) is a unique fungus in Taiwan, also known as Chang-chih or niu-chang-ku. The major bioactive constituents of AC are triterpenoids which reported to have various activities including anticancer and anti-inflammatory potential. There are two types comprising ergostane and lanostane of triterpenoids are reported from AC. The quality and content of active compounds in AC are dependent on harvest period, extraction methods and culture conditions. Thus, the quality control of active constituents in AC is of great importance. To evaluate the quality of AC, in this study, we developed an accurate and rapid LC-ESI-MS/MS analytical method for the determination of five bioactive triterpenoids namely dehydroeburicoic acid, 15α-acetyldehydrosulphurenic acid, antcin B, zhankuic acid C, antcin A, antcin C and antcin K. The optimized mass spectrometry operating conditions were as follows: negative ionization mode, scan spectra from m/z 150 to 700, nitrogen flow rate of 5 μl/min, capillary temperature at 400 °C, nebulizer gas pressure 15 arb and voltage 3 kV. An amplitude voltage of 39 - 42% and 30 - 35% was typically used for lanostane and ergostane triterpenoids, respectively, fragmentation in the ion trap auto-MS2 experiments.